An Atlas of Preimplantation Genetic DiagnosisThis color-illustrated atlas and clinical textbook by two respected authorities details the procedures for diagnosing genetic disease in gametes prior to fertilization and in embryos fertilized in vitro prior to uteral implantation. With over 200 full-color images, the book sets out the current methods used in oocyte and preembryo sampling along with the latest techniques in DNA and cytogenetic analysis in preimplantation development. Includes bibliographic references and index. Authors Verlinsky and Kuliev are well-known experts on this subject with two previous books to their credit. |
Contents
List of contributors | 2 |
Review of Methods and Experience in Preimplantation Genetic Diagnosis | 11 |
Micromanipulation and biopsy of polar bodies and blastomeres | 21 |
Nuclear transfer techniques for visualization of chromosomes in polar bodies and blastomeres | 27 |
Other editions - View all
Common terms and phrases
abnormal AF508 mutation amplification aneuploidies Assist Reprod Genet biopsy blastocyst blastomere blastomere biopsy bodies and blastomeres centromeric chromatid Chromatid error chromosome 18 chromosomes tested corresponding oocyte cumulus cells cytoplasm derivative of chromosome derived reciprocal translocation detected dish error in meiosis exon fertilization FISH analysis five-color FISH genotype gold arrow green arrow heterozygous holding pipette homozygous hybridization ICSI injection intron IVF patient linked markers meiosis metaphase chromosomes metaphase II metaphase II oocyte microneedle micropipette Midwest Scientific misdiagnosis mutant allele mutation nested PCR normal allele oocyte oocyte retrieved orange paternally derived PBI and PB2 PBI PB2 PCR product perivitelline space polar body analysis polar body PBI polar body removal pregnancy preimplantation genetic diagnosis preselection present primers red arrow restriction digestion resulting embryo second PB2 second polar body signal for chromosome single-gene disorders specific for chromosomes sperm thalassemia transfer trisomy Verlinsky white arrows zona pellucida zygote