High Performance Liquid Chromatography & Capillary Electrophoresis: Principles and PracticesHPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity.Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes.
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Contents
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Chapter 2 Separations in HighPerformance Liquid Chromatography | 24 |
Chapter 3 Instrumentation for HighPerformance Liquid Chromatography | 71 |
Chapter 4 Capillary Electrophoresis | 134 |
Chapter 5 Separation in Capillary Electrophoresis | 154 |
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Common terms and phrases
acetonitrile Adapted from Ref addition adsorption affinity chromatography amines amino acids Anal analysis analytical column anions applied band broadening baseline buffer Capillary Electrophoresis capillary wall cations Chem chiral Chromatogr compounds concentration conductivity detection decrease derivatization detector cell diameter diffusion effect efficiency electric field electrode electrolyte electromigration electroosmotic flow eluent elution enantiomers flow rate fluorescence fused silica gradient HPLC hydrophobic illustrated in Figure increase injector interactions interface ion-exchange ion-pair ionic strength length ligand linear mass spectrometry measurement MECC mechanism method micelle migration mixture mobile phase modes molecular weight molecules normal-phase particles peak area peak height permission polar polymer pore precolumn pressure Problem proteins pump range reagent Reprinted from Ref reservoir resolution result retention reversed-phase chromatography sample volume sensitivity separation shown in Figure solute solvent species standard stationary phase surface surfactant technique temperature theoretical plates tion typically velocity voltage wavelength zone