A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques
The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad. In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control.
What people are saying - Write a review
We haven't found any reviews in the usual places.
II SELECTED PROTOCOLS
Construction of Laboratory Equipment
InHouse Preparation of Reagents
Inventory for a PCR Laboratory
Good Laboratory Practice
Prevention of CrossContamination
PCR Troubleshooting Guide and Flowchart
Other editions - View all
10X Buffer 20 mM total agarose gel AMB/ATT analysis application assay cells Centrifuge the tubes Chlamydia trachomatis Clin coli color-coded pipettor containing cycle denaturation dengue virus detection diagnosis dNTPs epidemiology equipment ethidium bromide fragment gel electrophoresis gene genetic genome Grey Area hybridization ice bucket identification Incubate infections ingredients labeled tube laboratory Leishmania Leptospira membrane method MgCl2 Microbiol microcentrifuge tubes mineral oil minutes molecular biology mosquitoes Mycobacterium tuberculosis negative control nucleic acid nucleotides parasites pathogenic PCR amplification PCR Master Mix PCR products PCR reaction PCR-based Petri plate Phase pipet tips Plasmodium falciparum plastic polymerase chain reaction polymorphism preparing the PCR primers probe procedure protocol pullſ reagents restriction enzyme reverse transcriptase RT-PCR sample sensitivity sequence silica particles solution specific sterile strains supernatant Taq polymerase techniques temperature template thermocycler Transfer Trypanosoma cruzi U/ul vector Vibrio cholerae volume vortex water bath White Area Worksheet workshop