An Introduction to Genetic Engineering

Front Cover
Cambridge University Press, Feb 7, 2002 - Science - 292 pages
5 Reviews
Des Nicholl presents a new, fully revised, and expanded edition of his popular undergraduate-level textbook. The book retains many of the features of the original edition and still offers a concise technical introduction to the subject of genetic engineering. It is divided into three main sections: basic molecular biology, methods of gene manipulation, and modern applications of genetic engineering. Applications covered in the book include genomics, protein engineering, gene therapy, cloning, transgenic animals and plants, and bioethics. An Introduction to Genetic Engineering is essential reading for undergraduate students of biotechnology, genetics, molecular biology, and biochemistry.
 

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Contents

Introduction
1
12 Laying the foundations
3
13 First steps
4
14 What is in store?
6
The basis of genetic engineering
9
Introducing molecular biology
11
22 The structure of DNA and RNA
13
23 Gene organisation
16
75 Applications of the PCR
130
Selection screening and analysis of recombinants
132
81 Genetic selection and screening methods
133
82 Screening using nucleic acid hybridisation
138
83 Immunological screening for expressed genes
141
84 Analysis of cloned genes
143
Genetic engineering in action
151
Understanding genes and genomes
153

24 Gene expression
21
25 Genes and genomes
23
Working with nucleic acids
27
32 Handling and quantification of nucleic acids
29
33 Radiolabelling of nucleic acids
30
34 Nucleic acid hybridisation
33
36 DNA sequencing
35
The tools of the trade
43
42 DNA modifying enzymes
48
43 DNA ligase joining DNA molecules
52
The methodology of gene manipulation
55
Host cells and vectors
57
51 Host cell types
58
52 Plasmid vectors for use in E coli
60
53 Bacteriophage vectors for use in E coli
66
54 Other vectors
75
55 Getting DNA into cells
80
Cloning strategies
87
62 Cloning from mRNA
89
63 Cloning from genomic DNA
98
64 Advanced cloning strategies
106
The polymerase chain reaction
115
72 The methodology of the PCR
118
73 More exotic PCR techniques
123
74 Processing of PCR products
129
92 From genes to genomes
159
93 Genome sequencing
165
94 The human genome project
167
Genetic engineering and biotechnology
178
101 Making proteins
179
102 Protein engineering
183
103 Examples of biotechnological applications of rDNA technology
185
Medical and forensic applications of gene manipulation
197
112 Treatment using rDNA technology gene therapy
210
113 DNA profiling
215
Transgenic plants and animals
224
122 Transgenic animals
237
The other sort of cloning
247
132 Frogs and toads and carrots
250
133 A famous sheep the breakthrough achieved
253
134 Beyond Dolly
256
Brave new world or genetic nightmare?
259
142 Elements of the ethics debate
260
141 Does Frankensteins monster live inside Pandoras box?
262
Suggestions for further reading
263
Using the World Wide Web
266
Glossary
270
Index
287
Copyright

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Hairy Roots
Pauline M. Doran
Limited preview - 1997
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About the author (2002)

DESMOND NICHOLL is Senior Lecturer in the Department of Biological Sciences, The University of Paisley, Scotland

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