Bancroft's Theory and Practice of Histological Techniques E-BookThis is a brand new edition of the leading reference work on histological techniques. It is an essential and invaluable resource suited to all those involved with histological preparations and applications, from the student to the highly experienced laboratory professional. This is a one stop reference book that the trainee histotechnologist can purchase at the beginning of his career and which will remain valuable to him as he increasingly gains experience in daily practice. Thoroughly revised and up-dated edition of the standard reference work in histotechnology that successfully integrates both theory and practice.Provides a single comprehensive resource on the tried and tested investigative techniques as well as coverage of the latest technical developments. Over 30 international expert contributors all of whom are involved in teaching, research and practice.Provides authoritative guidance on principles and practice of fixation and staining. Extensive use of summary tables, charts and boxes.Information is well set out and easy to retrieve. Six useful appendices included (SI units, solution preparation, specimen mounting, solubility). Provides practical information on measurements, preparation solutions that are used in daily laboratory practice. Color photomicrographs used extensively throughout. Better replicates the actual appearance of the specimen under the microscope. Brand new co-editors. New material on immunohistochemical and molecular diagnostic techniques.Enables user to keep abreast of latest advances in the field. |
Contents
1 Managing the laboratory | 1 |
2 Safety and ergonomics in the laboratory | 11 |
3 Light microscopy | 37 |
4 Fixation of tissues | 69 |
5 The gross roomsurgical cutup | 95 |
6 Tissue processing | 105 |
8 Plastic embedding for light microscopy | 139 |
9 How histological stains work | 157 |
16 Bone | 317 |
17 Techniques in neuropathology | 353 |
18 Immunohistochemical techniques | 381 |
19 Immunofluorescent techniques | 427 |
20 Immunohistochemistry quality control | 435 |
21 Molecular pathology | 455 |
22 Transmission electron microscopy | 493 |
23 Quantitative data from microscopic specimens | 539 |
10 The hematoxylins and eosin | 173 |
11 Connective and mesenchymal tissues with their stains | 187 |
12 Carbohydrates | 215 |
13 Pigments and minerals | 239 |
14 Amyloid | 271 |
15 Microorganisms | 291 |
Appendices Diagnostic Appendices | 561 |
Appendices Technical Appendices | 585 |
| 605 | |
| 609 | |
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Common terms and phrases
acetic acid alcian blue alcohol amyloid amyloidosis antibody antigen antigen retrieval aqueous biopsies birefringence block bone buffer calcium cells chemical chloride chromosome Clinical collagen color components concentration Congo red connective tissue counterstain cytoplasm Dako decalcified Dehydrate demonstration deposits detection diagnostic differentiation dilution disease distilled water embedding enzyme eosin ethanol fibers fibrils filter fixation fixative fluorescence formaldehyde formalin frozen sections fuchsin hematoxylin histochemical Histochemistry Histochemistry and Cytochemistry histological hybridization immunohistochemical immunohistochemistry Incubate Journal of Histochemistry labeled laboratory light melanin membrane method methyl microscope microtome microwave minutes molecular molecules mount mucins nuclei nucleic acids oxidation paraffin sections Pathology pigment plastic potassium preparation primary antibody probe procedure processing protein proteoglycans reaction reagents removed resin Rinse room temperature routine samples silver slides sodium solution specific specimen staining sulfate tap water technique tion tissue sections tumors Wash xylene