Biochemical Methods

Front Cover
New Age International, 1996 - Biochemistry - 272 pages
Biochemical Methods Are Used In All Branches Of Biological Sciences And Agriculture Is No Exception. Research In Various Branches Of Agriculture Viz. Plant Physiology, Plant Pathology, Agricultural Microbiology Seed Technology Plant Genetics And Entomology Requires One Or The Other Biochemical Methods. A Researcher Has To Refer Many Journals And Books Before He Could Get To The Right Procedure For His Experiment. This Book On Biochemical Methods Attempts To Give Often Used Methods In A Single VolumeThe Book, Divided Into 13 Chapters Contains 115 Procedures. The Chapters Are Carbohydrates, Lipids, Proteins, Nucleic Acids, Vitamins, Enzymes, Nitrogen Fixation Antinutritional Factors, Plant Hormones, Pigments, Phenols Cell Fractionation And Separation Techniques. Each Procedure Is Divided Into Introduction, Principle, Materials, Procedure And Calculation. At The End Of Each Procedure References For Additional Reading Are Provided. Important Precautions, Warnings And Tips Are Given In The Notes Section.The Methods Elaborated In The Book Will Be Useful For Conducting Practical Classes At The Undergraduate And Postgraduate Levels In Science Colleges And Universities. This Manual Will Be A Bonanza For The Research Workers In Plant Sciences Since It Includes Procedures From The Classical Microkjeldahl Nitrogen Estimation To The Modern Southern Blotting Technique.
 

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Contents

CARBOHYDRATES
1
5
2
Determination of glucose by glucose oxidase method
7
2
20
LIPIDS
22
5
87
ENZYMES
98
8
105
Extraction of plant DNA
154
Estimation of RNA
160
1
163
2
171
VITAMINS
179
PIGMENTS
187
PHENOLICS
193
ANTINUTRITIONALFACTORS
202

9
112
Combined assay
128
2
144
NUCLEICACIDS
148
1
151
1
209
Fractionation of cellular components
235
APPENDIX
241
INDEX
251
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