Biochemical MethodsBiochemical Methods Are Used In All Branches Of Biological Sciences And Agriculture Is No Exception. Research In Various Branches Of Agriculture Viz. Plant Physiology, Plant Pathology, Agricultural Microbiology Seed Technology Plant Genetics And Entomology Requires One Or The Other Biochemical Methods. A Researcher Has To Refer Many Journals And Books Before He Could Get To The Right Procedure For His Experiment. This Book On Biochemical Methods Attempts To Give Often Used Methods In A Single VolumeThe Book, Divided Into 13 Chapters Contains 115 Procedures. The Chapters Are Carbohydrates, Lipids, Proteins, Nucleic Acids, Vitamins, Enzymes, Nitrogen Fixation Antinutritional Factors, Plant Hormones, Pigments, Phenols Cell Fractionation And Separation Techniques. Each Procedure Is Divided Into Introduction, Principle, Materials, Procedure And Calculation. At The End Of Each Procedure References For Additional Reading Are Provided. Important Precautions, Warnings And Tips Are Given In The Notes Section.The Methods Elaborated In The Book Will Be Useful For Conducting Practical Classes At The Undergraduate And Postgraduate Levels In Science Colleges And Universities. This Manual Will Be A Bonanza For The Research Workers In Plant Sciences Since It Includes Procedures From The Classical Microkjeldahl Nitrogen Estimation To The Modern Southern Blotting Technique. |
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100mL water absorbance acetic acid acetone acrylamide add 1mL agarose alcohol aliquots alkaline amino acids anthrone antibody antigen antiserum assay Biochem blank blotting bromine Calculation capsaicin cells cellulose Centrifuge chloride chromatography colour column compounds concentration conical flask containing Cool destaining dilute Dissolve distilled water EDTA electrophoresis elution enzyme Enzyme Extract estimation ethanol ethidium bromide fatty acids filter paper filtrate fractions glass plate glucose Glutamate heat homogenate hydrogen hydrolysed Incubate layer litre Materials measured method molecular molecules NaCl NADH NaOH nitrate nitrocellulose nitrogen oxidase oxidation pectin pellet petroleum ether phenol phosphate buffer pH plant plasmid potassium precipitate Prepare PRINCIPLE Procedure 1 Pipette protein quantity READINGS reagent remove residue room temperature Sadasivam sample separation shake sodium hydroxide solution solvent staining standard graph starch substrate sucrose sugars sulphate supernatant test tube tissue transfer Tris-HCl pH volume volumetric flask Wash weight Whatman