Capillary Electrophoresis Guidebook: Principles, Operation, and Applications

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Springer Science & Business Media, 1996 - Science - 349 pages
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Capillary Electrophoresis Guidebook offers both newcomers and experienced research workers hands-on guidance to performing capillary electrophoresis. It provides sufficient practical advice to permit you to develop and optimize your own separations, along with extensive troubleshooting sections to overcome practical difficulties. The book contains operating instructions for standard commercially available instruments and includes guidelines for activities such as changing capillaries, method development, quantitative procedures, optimizing sensitivity, and the validation of methods. Review chapters written by leading experts discuss micellar electrokinetic capillary chromatography, capillary gel electrophoresis, advanced sampling techniques, and electrochromatography. Important application areas such as the analysis of proteins, peptides, amino acids, pharmaceuticals, chiral compounds, and nucleic acids are also treated.
 

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Contents

Fundamentals of Capillary Electrophoresis Theory
3
Standard Commercial Instrument Description
15
Typical Operating Procedures
21
Method DevelopmentOptimization
29
Quantitation Procedures
49
Optimization of Precision in Quantitative Analysis
61
Optimization of Sensitivity
71
Method Validation
83
Micellar Electrokinetic Chromatography
125
Capillary Gel Electrophoresis
157
Chiral Separations by Capillary Electrophoresis
171
Capillary Electrochromatography
197
Application and Limits of Sample Stacking in Capillary Electrophoresis
211
Analysis of Bases Nucleosides and Oligonucleotides
227
Application of Capillary Electrophoresis to Pharmaceutical Analysis
265
Separation of Peptides and Protein Digests by Capillary Electrophoresis
285

Fraction Collection
99
Troubleshooting
105
Quick Guide to Running a Successful Separation
119
Additional Application Areas of Capillary Electrophoresis
309
Index
345
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