Carleton's Histological Technique

70 per cent absolute alcohol Alcian blue alum block bone buffer calcium celloidin cells cellulose nitrate cent acetic acid cent alcohol cent aqueous cent formal-saline cent sodium chloride clear in xylene cm³ colour compounds containing Counterstain coverslip cryostat cryostat sections cutting cytoplasm decalcification Dehydrate demonstration differentiation diluted Dissolve distilled water dyes electron microscope embedding emulsion enzyme eosin examination fibres film filter fixation fixed fluid fluorescence formalin fresh frozen sections glycerol granules haematoxylin histochemical histological hydrochloric acid impregnation iodine knife light lipids material methyl microtome mixture mordant mount mucopolysaccharides mucosubstances myelin neutral nuclei osmium tetroxide oxidation paraffin wax Path phosphate picric acid pigment potassium PREPARATION protein reaction reagent removed RESULTS Rinse in distilled sections to water silver slide smears soluble specimen Stain Technol stained sections staining methods staining solution substrate sulphate synthetic resin medium tap water technique temperature thick thin Wash in running xylene