Chromatographic MethodsThis book provides a unified and balanced introduction to the general theory of chromatography, followed by a detailed treatment of the principles and practice of all the major techniques currently employed in the industrial and academic sectors. It is written as a broad introduction to the subject for mid to advanced undergraduates in chemistry, pharmacy, biochemistry, and is suitable for students following the now quite numerous Masters degrees in instrumental analysis. The book has been updated to incorporate advances of the last ten years, and it contains around 50% new or revised material. |
Contents
| 1 | |
| 17 | |
Planar chromatography | 44 |
Liquid phase chromatography on open columns | 117 |
Gas chromatography | 165 |
High performance liquid chromatography | 258 |
References | 362 |
Chromatography and spectroscopic techniques | 366 |
Model or practical experiments in chromatographic techniques | 426 |
electrophoresis of proteins | 435 |
derivatives by capillary GC and GCMS | 484 |
| 509 | |
Illustrative problems in chromatography | 510 |
Glossary of chromatographic terms | 525 |
Table of chromatography symbols | 546 |
Processing chromatographic data | 399 |
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Common terms and phrases
acetate achieved adsorbent adsorption alcohol alumina amino acids Anal analysis analyte anion application aqueous band broadening bonded buffer calculated capillary columns capillary electrophoresis carbon carrier gas cation cellulose Chem column efficiency column temperature components compounds concentration containing derivatisation detection detector response dextran electron electrophoresis eluant Figure film flow-rate fluorescence Gas Chromatography gradient elution HPLC hydrogen injection instruments interface internal standard ion exchange ionic ionisation layer chromatography linear liquid material methanol method methyl mixture ml min¯¹ mobile phase molecules non-polar obtained optimisation packed columns paper paper chromatography particle peak area peak width polar polymer pore pressure proteins pump quantitative range ratio reagent resin resolution retention reverse phase sample sensitivity separation silica gel solution solvent sorbent specific spherical stationary phase surface technique theoretical plates thin layer thin layer chromatography tube valve velocity volume wavelength WCOT WCOT columns