Electron Microscopy: Methods and Protocols

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Springer Science & Business Media, 2007 - Medical - 608 pages
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Hands-on experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. Electron Microscopy Methods and Protocols, Second Edition, offers both newcomers and established researchers across experimental biology and medicine wanting to expand their repertoire a gold-standard laboratory manual of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual. New to this edition are sections covering transmission electron microscopy (TEM), and scanning electron microscopy (SEM).
 

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Contents

Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells
1
Processing Biological Tissues for Ultrastructural Study
19
Processing Plant Tissues for Ultrastructural Study
35
MicrowaveAssisted Processing and Embedding for Transmission Electron Microscopy
47
Ultramicrotomy for Biological Electron Microscopy
67
Poststaining Grids for Transmission Electron Microscopy Conventional and Alternative Protocol
97
Negative Staining of Thinly Spread Biological Samples
107
Recent Advances in HighPressure Freezing Equipment and SpecimenLoading Methods
143
Electron Crystallography of Membrane Proteins
331
Cryoelectron Microscopy of Icosahedral Virus Particles
345
ThreeDimensional Reconstruction of Chromosomes Using Electron Tomography
365
WholeMount Immunoelectron Tomography of Chromosomes and Cells
387
ThreeDimensional Cryotransmission Electron Microscopy of Cells and Organelles
407
Electron EnergyLoss Spectroscopy as a Tool for Elemental Analysis in Biological Specimens
431
Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specimens
449
Variable Pressure and Environmental Scanning Electron Microscopy Imaging of Biological Samples
467

Cryoultramicrotomy Cryoelectron Microscopy of Vitreous Sections
175
CellFree Extract Systems and the Cytoskeleton Preparation of Biochemical Experiments for Transmission Electron Microscopy
199
Electron Microscopy In Situ Hybridization Tracking of DNA and RNA Sequences at High Resolution
213
Correlative Light and Electron Microscopy Using Immunolabeled Resin Sections
229
Cryosectioning Fixed and Cryoprotected Biological Material for Immunocytochemistry
257
FluorescenceIntegrated Transmission Electron Microscopy Images Integrating Fluorescence Microscopy With Transmission Electron Microscopy
291
Quantitative Immunoelectron Microscopy Alternative Ways of Assessing Subcellular Patterns of Gold Labeling
309
Cryoplaning Technique for Visualizing the Distribution of Water in Woody Tissues by Cryoscanning Electron Microscopy
497
XRay Microanalysis in the Scanning Electron Microscope
507
Botanical XRay Microanalysis in Cryoscanning Electron Microscopy
529
Static Secondary Ion Mass Spectrometry for Biological and Biomedical Research
543
Using SIMS and MIMS in Biological Materials Application to Higher Plants
569
Index
593
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