A clear and authoritative guide to the principles and practice of the utilization of enzymes in biotechnology is presented in this textbook. Enzymes have increasingly important applications in the food and pharmaceutical industry, in medicine, and as biosensors. The reader is provided with a balanced account of the practicalities and problems involved in harnessing this technology. While the underlying principles are fully explained, the emphasis throughout is on the practical and economic aspects of enzyme usage. The book devotes in-depth coverage to the uses of enzymes in their free and immobilized state. This book is intended for students of biotechnology and biochemical engineering.
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Fundamentals of enzyme kinetics
Enzyme preparation and use
The preparation and kinetics of immobilised enzymes
Effects of solute diffusion on the kinetics of immobilised
The largescale use of enzymes in solution
Immobilised enzymes and their use
Recent advances in enzyme technology
Future prospects for enzyme technology
a-amylase achieved acid aqueous batch binding biocatalyst biosensors biphasic systems bulk catalysed cause cell cellulose centrifuge changes charged coenzymes concentration gradient cost covalent CSTR curve denaturation detergents diameter diffusion diffusional diffusional control electrode enzyme activity enzyme preparations enzyme technology enzyme-catalysed reaction equation equilibrium constant ester Figure flow rate free enzyme fructose glucoamylase glucose isomerase glucose oxidase groups heat hydrogen ions hydrolysis hydrophobic immobilised enzyme immobilised-enzyme inactivation increase industrial inhibitor invertase involving ionic strength ionisation kinetic Kmax lactase lipases maltose material membrane method microbial microenvironment microenvironmental molecular weight molecules oligosaccharides organic phase oxidase oxygen particles partition coefficient peptide polymers porous potential proteases protein pullulanase purification rate of reaction reactants reaction scheme reactor reduced relative removed residues reversible reactions soluble solution solvents specific stabilisation starch substrate and product substrate concentration substrate modulus substrate stream sucrose surface synzyme syrup temperature utilised values