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70 per cent absolute alcohol acetic acid acetone antibody balsam or D.P.X. block Bring sections buffer calcium Canada balsam celloidin cells cent alcohol cent aqueous cent sodium chloride colour condenser containing Counterstain coverslip cytoplasm D.P.X. Results decalcifying Dehydrate demonstration Differentiate dissolved distilled water dyes embedding enzyme eosin fibres filter paper fixation fixed fluid fluorescent formalin formol saline frozen sections fuchsin gelatin gives glass glycerin granules haematoxylin hydrochloric acid impregnation inch knife lens lipids membrane method methyl microscope microtome minutes mitochondria mount in Canada mucin myelin neutral red nuclei object osmium tetroxide paraffin sections paraffin wax Perspex picric acid pigment potassium dichromate prepared protein reaction remove Rinse in distilled routine Schiff reagent sections to water silver slide smears Special reagents required specimen stage staining solution substrate sulphate tap-water technique thickness Transfer tube usually violet Wash in running Wash in water xylol