High-performance Thin-layer Chromatography for the Analysis of Medicinal Plants

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Thieme, 2007 - Medical - 264 pages

With practical examples of how to establish the identity and quality of botanical products

High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants presents the theoretical and technical information needed to perform reliable and reproducible high-performance thin-layer chromatography (HPTLC) to establish the identity, purity, quality, and stability of raw materials, extracts, and finished botanical products.

The text provides a complete overview of the technique and common applications of HPTLC in herbal analysis. It will help the analyst answer questions such as:

  • Am I paying for a high-quality material, but getting a cheap adulterant?
  • Is this raw material worth its price?
  • Does this product comply with the claim on its label?
  • Has the composition of this product changed after being on the shelf for more than a year?

Practical examples provided by renowned experts help the reader gain a firm understanding of HPTLC methodologies. More than 300 full-color illustrations aid comprehension of complex concepts and easy-to-reference text boxes provide summaries of key information.

This book is essential for analysts, quality assurance professionals, and regulators seeking a comprehensive text on how to use HPTLC to determine whether botanicals comply with current, good manufacturing practices. It will also benefit students in pharmacognosy, phytopharmacy, pharmaceutical biology, and analytical chemistry programs.

 

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Contents

Introduction
1
What Is Chromatography?
6
What Is TLC?
11
Modern TLC
19
Theoretical Concepts
22
Special Aspects of TLC
33
The Stationary Phase
42
The Mobile Phase
48
Typical Applications in Herbal Analysis
129
HPTLC Analysis of Raw Material
132
HPTLC Analysis of Herbal Drug Preparations and Products
160
Method Development
175
Method Development in Practice
179
Validation
193
Validation of Qualitative HPTLC Methods
206
Validation of Quantitative HTPLC Methods
210

The Chromatographic Result in TLC
55
Densitometry
61
Practical Aspects of Modern TLC
67
Sample Preparation
68
Selecting the Stationary Phase
73
Sample Application
92
Chromatogram Development
97
Derivatization
110
Densitometry
116
Reporting and Record Keeping
121
TLC Software
125
Practical Recommendations
127
The Final Word
214
List of Abbreviations
215
Glossary of TLC Terms
218
Definitions
224
Standard Operating Procedure for HPTLC of Herbal Raw Materials and Preparations
227
Resources
231
Common Mobile Phases for the Screening of Unknown Herbal Drugs
233
Most Common Nonspecific Derivatization Reagents for Analysis of Herbal Drugs
234
Worksheet
238
Validation of Method for Identification of Eleuthero by HPTLC Fingerprint
240
Index
259
Copyright

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About the author (2007)

Assistant Head of Quality Control, SwissCo Services AG, Sisseln, Switzerland

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