Histopathologic Technic and Practical Histochemistry
McGraw-Hill, 1976 - Histochemistry - 942 pages
Micrscopy. Equipment. Fixation. Sctioning. General Staining ans mounting procedures. Stains and Fluorochromes. Nuclei, nucleic acids, general oversight methods. Chemical end groups. Cytopasmic ganules and organelles. Enzymes. Endogenous pigments. Metals, anions, exogenous pigments. Lipids. Polysaccharides, mucins. connective tissue fibers and membranes. Smear preparations, bacteria, protozoa, and other parasites. Glia and never cells and fibers. Hard tissues, decalcification. Various special procedures. Bufffers and buffers tables, normal acids and alkalies.
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acetic acid acetone activity added alcohol aldehyde alum appears aqueous basic blocks blue brown buffer cells changes chloride clear color completely concentration containing counterstain Cytochem cytoplasm dark Dehydrate demonstration differentiation dilute Dissolve distilled water drop effect electron embedding employed enzyme ethanol extraction filter fixation fixed fluid fluorescence formaldehyde formalin fresh frozen sections fuchsin gelatin gives glass glucose glycerol granules green groups hematoxylin Histochem Immerse incubation iron lead less light Lillie lipids material medium method methyl microscopic mixture mount neutral noted nuclei observed occurs oxidation paraffin Pathol periodic acid phosphate pigment potassium prepared prevent procedure protein reaction reagent recommended reduced remove reported resin RESULTS Rinse salt saturated Schiff silver slides sodium solution specific Stain Technol staining step studies substrate Table technic temperature tissue usual violet Wash xylene yellow