Immobilization of Enzymes and Cells

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Springer Science & Business Media, 2006 - Science - 449 pages
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The continuing rapid progress in work designed to improve the functional properties of enzymes and cells as industrial catalysts has led to this revised, updated, and expanded new edition of the warmly received initial edition of Immobilization of Enzymes and Cells. This long-awaited second edition contains new and simplified protocols useful for industrial applications, novel techniques that will prove useful now or in the near future, and protocols for the preparation of immobilized derivatives suitable for a wide variety of nonconventional reaction media. The authors also offer tools for the development of new immobilization techniques, methods for preparing immobilized derivatives for therapeutic and industrial use, and new chemical reactors designed to overcome the limitations of immobilized derivatives. The emphasis is on improving enzyme and cell properties via very simple immobilization protocols, along with the development of new and better methods. The protocols follow the successful Methods in Biotechnology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Immobilization of Enzymes and Cells, Second Edition, affords biochemists, biotechnologists, and biochemical engineers a practical review of all the latest methods and tools-as well as optimized conventional techniques-needed to carry out successful research involving immobilizing enzymes and cells.
 

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Contents

Immobilization of Enzymes as the 21st Century Begins An Already Solved Problem or Still an Exciting Challenge?
1
Immobilization of Enzymes A Literature Survey
15
CrossLinked Enzyme Aggregates
31
ImmobilizationStabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups
47
Glutaraldehyde in Protein Immobilization A Versatile Reagent
57
Practical Protocols for Lipase Immobilization Via SolGel Techniques
65
Encapsulation of Enzymes Using Polymers and SolGel Techniques
77
Design of Smart Biocatalysts Immobilization of Enzymes on Smart Polymers
87
Immobilization of Enzymes on Electrodes
239
Immobilization of Enzymes for Use in Organic Media
251
Immobilization of Enzymes for Use in Ionic Liquids
257
Immobilization of Enzymes for Use in Supercritical Fluids
269
Immobilized Enzymes for Biomedical Applications
283
Characterization of Immobilized Enzymes by Microcalorimetry
295
Use of Immobilized Biocatalysts in Fluidized Bed Format
311
TaylorCouette Vortex Flow in Enzymatic Reactors
321

Affinity Immobilization of Tagged Enzymes
97
Bioaffinity Immobilization
107
OneStep Purification Immobilization and Stabilization of PolyHistidineTagged Enzymes Using Metal ChelateEpoxy Supports
117
Stabilization of Multimeric Enzymes Via Immobilization and Further CrossLinking With AldehydeDextran
129
Purification Immobilization Hyperactivation and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports
143
Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel AminoEpoxySepabeads
153
Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports
163
Stabilization of New Imprint Property of Glucose Oxidase in Pure Aqueous Medium by CrossLinkedImprinting Approach
175
Reversible Covalent Immobilization of Enzymes Via Their Thiol Groups
185
Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers
205
Immobilization of Enzymes on Magnetic Particles
217
Immobilization of Proteins on Gold Surfaces
229
A Novel Immobilization Method for Entrapment LentiKats
333
Encapsulation of Cells in Alginate Gels
345
Immobilization of Cells on Polyurethane Foam
357
Immobilization of Cells With Transition Metal
367
Immobilization of Microalgae Nirupama Mallick
373
Bioluminescence in Immobilized Cells for Biomass Detection and Biosensor Applications
393
A Proteomic Approach to Biofilm Cell Physiology
403
Encapsulation of Bacteria for Biodegradation of Gasoline Hydrocarbons
415
Biomedical Applications of Immobilized Cells
427
Index
439
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