Laboratory Manual on Fundamental Principles of Bacteriology

acid Adjust the reaction agar plate agar slant culture agar to cool agar to harden Allow the agar anaerobic Arnold sterilizer aureus autoclave autoclave for 15 Bacillus subtilis bacteria bromocresol purple bromothymol blue cells cent solution coliform colonies color cool to 50°C count cover slips crystal violet culture of Bacillus culture of Escherichia decolorization dilution blank Dispense in test distilled water Escherichia coli Exercise flame flask following table Gram stain growth Incubate the tubes indicator solution Invert the plates lactose loopful Meat extract medium Melt method methylene blue milk nitrite nutrient agar slant nutrient broth culture oil-immersion objective organisms Peptone Petri dishes phenol pipettes plates and incubate Procedure reaction to pH Record your results Required sample sodium chloride sodium hydroxide sterile Sterile 1-ml sterile Petri dishes Streak sucrose sufficient distilled water surface tap water temperature test tubes Transfer 1 ml tryptone tubes at 37°C tubes of nutrient vulgaris