Manual of Histologic Staining Methods ; of the Armed Forces Institute of Pathology. Edited by Lee G. Luna
Lee G. Luna
Blakiston Division, McGraw-Hill, 1968 - Histology, Pathological - 258 pages
Preparation of tissues; Processing of tissue; Preparation of sections; Routine staining procedures; Special techniques; Methods for connective tissue; Methods for connective tissue; Methods for cytoplasmic granules; Methods for hematologic and nuclear elements; Methods for fats and lipids; Methods for carbohydrates and mucoproteins; Methods for pigments and minerals; Methods for nerve cells and fibers; Methods for bacteria, fungi, and inclusion bodies.
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Preparation of Tissues
Processing of Tissue
Preparation of Sections
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1.0 gm Distilled 100.0 ml STAINING 15 minutes 24 hours 30 minutes absolute alcohol acetic acid solution Alcian blue Aniline aqueous Armed Forces Institute block bone BUFFER SOLUTION buffered neutral formalin celloidin cells clear in xylene Copyright by Williams Counterstain crystals Cut paraffin sections Cytoplasm decalcifying Dehydrate in 95 Deparaffinize and hydrate Differentiate dips Distilled water 100.0 eosin fibers FIXATION fixative frozen sections Glacial acetic acid gm Alcohol gm Distilled water hydrate to distilled Hydrochloric acid Institute of Pathology knife lithium carbonate METHOD Methyl microns ml Alcohol ml Glacial acetic ml STAINING PROCEDURE Mount with Permount nitrocellulose Permount or Histoclad Phloxine Phosphomolybdic acid Phosphotungstic acid picric acid Place Potassium ferrocyanide REFERENCE remove RESULTS Rinse in distilled running water Silver nitrate SILVER NITRATE SOLUTION Sodium thiosulfate solution for 30 SOLUTION STOCK specimen sulfate tap water TECHNIQUE three changes tissue Wash in running Wash in tap WEIGERT'S IRON HEMATOXYLIN Williams and Wilkins