Metabolic Engineering: Principles and Methodologies
Academic Press, Oct 17, 1998 - Science - 725 pages
Metabolic engineering is a new field with applications in the production of chemicals, fuels, materials, pharmaceuticals, and medicine at the genetic level. The field's novelty is in the synthesis of molecular biology techniques and the tools of mathematical analysis, which allow rational selection of targets for genetic modification through measurements and control of metabolic fluxes. The objective is to identify specific genetics or environmental manipulations that result in improvements in yield and productivities of biotechnological processes.
Key features of the book are pathway integration and the focus on metabolic flux as a fundamental determinant of cell physiology. The book keeps mathematical complexity to a minimum, and provides a glossary of biological terms to facilitate use of the book by a broader spectrum of readers. A web page exists to communicate updates of the codes and homework problems.
* Demonstrates metabolic engineering in action with numerous examples of pathway modification
* Includes methods for identifying key enzymes in metabolic networks
* Contains a comprehensive review of metabolic biochemistry
* Discusses metabolic regulation at the gene, enzyme, operon, and cell levels
* Explains concepts of stoichiometry, kinetics, and thermodynamics of metabolic pathways
* Minimizes mathematical complexity
* Links to a Web page to communicate updates of the software code and homework problems
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Chapter 3 Comprehensive Models for Cellular Reactions
Chapter 4 Material Balances and Data Consistency
Chapter 5 Regulation of Metabolic Pathways
Metabolic Engineering in Practice
Chapter 7 Metabolic Pathway Synthesis
Chapter 8 Metabolic Flux Analysis
Chapter 9 Methods for the Experimental Determination of Metabolic Fluxes by Isotope Labeling ...
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AcCoA acetate acetyl-CoA amino acids amplification bacteria balance biochemical biomass biosynthesis Biotechnology branch point calculated carbon catabolic cell cellular cerevisiae complex compounds conversion dehydrogenase derived determined elasticity coefficients enzymatic enzyme activity equations equilibrium estimates ethanol example experimental FCCs fermentation flux analysis flux control coefficients formation free energy function g DW genes genetic gFCCs Gibbs free energy glucose glutamate glutamicum inhibition inhibitor intracellular metabolites isotopomers kernel kinase kinetics labeled lactate linear link metabolite lysine matrix measurements membrane metabolic engineering metabolic flux metabolic networks metabolic pathways metabolic products metabolite metabolite concentrations molecules mutant NADH NADPH operon overall oxaloacetate oxidation parameters perturbation phosphorylation protein proton pyruvate ratio reaction rate recombinant regulation specific growth rate steady step stoichiometric coefficients stoichiometry strain substrate succinate synthesis Table TCA cycle thermodynamic tion transport tryptophan uptake vector xylose yield