Metabolic Engineering: Principles and Methodologies

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Elsevier, Oct 17, 1998 - Technology & Engineering - 725 pages
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Metabolic engineering is a new field with applications in the production of chemicals, fuels, materials, pharmaceuticals, and medicine at the genetic level. The field's novelty is in the synthesis of molecular biology techniques and the tools of mathematical analysis, which allow rational selection of targets for genetic modification through measurements and control of metabolic fluxes. The objective is to identify specific genetics or environmental manipulations that result in improvements in yield and productivities of biotechnological processes.

Key features of the book are pathway integration and the focus on metabolic flux as a fundamental determinant of cell physiology. The book keeps mathematical complexity to a minimum, and provides a glossary of biological terms to facilitate use of the book by a broader spectrum of readers. A web page exists to communicate updates of the codes and homework problems.

  • Demonstrates metabolic engineering in action with numerous examples of pathway modification
  • Includes methods for identifying key enzymes in metabolic networks
  • Contains a comprehensive review of metabolic biochemistry
  • Discusses metabolic regulation at the gene, enzyme, operon, and cell levels
  • Explains concepts of stoichiometry, kinetics, and thermodynamics of metabolic pathways
  • Minimizes mathematical complexity
  • Links to a Web page to communicate updates of the software code and homework problems

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This book's first author should be Gregory Stephanopoulos

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i want this book FOR FREE ^_^


Chapter 1 The Essence of Metabolic Engineering
Chapter 2 Review of Cellular Metabolism
Chapter 3 Comprehensive Models for Cellular Reactions
Chapter 4 Material Balances and Data Consistency
Chapter 5 Regulation of Metabolic Pathways
Metabolic Engineering in Practice
Chapter 7 Metabolic Pathway Synthesis
Chapter 8 Metabolic Flux Analysis
Chapter 10 Applications of Metabolic Flux Analysis
Chapter 11 Metabolic Control Analysis
Chapter 12 Analysis of Structure of Metabolic Networks
Chapter 13 Flux Analysis of Metabolic Networks
Chapter 14 Thermodynamics of Cellular Processes

Chapter 9 Methods for the Experimental Determination of Metabolic Fluxes by Isotope Labeling ...

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About the author (1998)

Gregory Stephanopoulos is a Professor of Chemical Engineering at MIT. He received his B.S. from the National Technical University of Athens, his M.S. from the University of Florida and his Ph.D. from the University of Minnesota, all in Chemical Engineering. Upon graduation, he joined the Chemical Engineering Faculty of the California Institute of Technology, where he served as Assistant and Associate Professor until 1985. In 1985 he was appointed Professor of Chemical Engineering at MIT where he has been ever since.Stephanopoulos' work has appeared in more than 150 publications and 7 patents. He has been recognized with the Dreyfus Foundation Teacher Scholar Award (1982), Excellence in Teaching Award (1984), and Technical Achievement Award of the AIChE (1984). He has been a Presidential Young Investigator and the Chairman of the Food Pharmaceutical & Bioengineering Division of the American Institute of Chemical Engineers (1992). In 1992 he was a Visiting Professor at the International Research Center for Biotechnology at Osaka University and was elected a Founding Fellow of the American Institute for Medical and Biological Engineering. In 1996 he chaired the first Conference on Metabolic Engineering and gave the inaugural Bayer Lecture on Biochemical Engineering at the University of California at Berkeley. He was honored with the FPBE Division Award at AIChE in 1997.