Methods for General and Molecular BacteriologyA major revision of the classic manual from ASM. This is the long awaited revision of ASM′s extremely popular title, Manual of Methods for General Bacteriology (1981). The goal of the book remains to provide a compact but thorough compendium of reliable methods of working with many different kinds of bacteria in laboratory settings. New to this edition is the recognition of the dramatic role of molecular biological techniques and their impact on bacteriology. |
Contents
Electron Microscopy Terry J Beveridge Terry J Popkin and Roger M Cole | 42 |
Cell Fractionation G Dennis Sprott Susan F Koval and Carl A Schnaitman | 72 |
AntigenAntibody Reactions Joseph S Lam and Lucy M Mutharia | 104 |
Copyright | |
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acetate acid agar agar plate agarose anaerobic antibiotic antibody antigen assay autoclaving bacte bacteria bacterial cells Bacteriol bacterium broth buffer centrifugation Chapter chemical chromosomal cloning colonies concentration containing culture density detection dilution Distilled water DNA polymerase donor EDTA electron electrophoresis enzyme Escherichia coli ethanol extract film filter flask fragments gene genetic glass gradient gram-negative gram-positive growth Incubate Inoculate insertion isolation labeled laboratory liquid lysate lysis medium membrane method Microbiol Microbiology microscopy mixture molecular mutagenesis mutants NaCl nutrient organisms oxygen Pasteur pipette pellet phage phase plasmid plasmid DNA plates polymerase polymerase chain reaction preparation primer procedure protein protoplasts purification reaction reagents recipient remove Resuspend S-layer sample selection sequencing sodium solution specific specimen sterile strain substrate sucrose supernatant surface suspension technique temperature tion transduction transfer transposon tube vectors vials volume wash wt/vol