Molecular Biology and BiotechnologyJohn M. Walker, Ralph Rapley The 3rd Edition of this immensely popular textbook brings readers up to date with the latest advances in this rapidly developing field. It has been thoroughly revised and expanded, combining entirely new chapters on current hot topics with existing chapters that have been rewritten and extended. Molecular Biology and Biotechnology 3rd Edition provides real breadth of coverage, taking account of both molecular biology and industrial applications. It also gives in depth coverage of key areas, resulting in a uniquely comprehensive publication. Highlights of this 3rd Edition include increased coverage of the cloning of yeast and animal cells and plant genetic engineering, and new coverage of polymerase chain reactions, DNA fingerprinting, transgenesis and vaccine development, reflecting the rapid growth of interest in these areas. The book presents the information in an easily assimilated form and makes an ideal undergraduate text. It will be of particular interest to students of biology and chemistry, as well as to scientific workers from outside the field who need a rapid introduction to the subject. Molecular Biology and Biotechnology primarily has a teaching function and as such should prove invaluable. |
Contents
Fermentation Technology | 1 |
Electrophoresis of Nucleic Acids | 30 |
Recombinant DNA Technology | 67 |
Production of Gene Probes | 95 |
The Expression of Foreign DNA in Bacteria | 125 |
Yeast Cloning and Biotechnology | 155 |
Cloning Genes in Mammalian Celllines | 177 |
Plant Biotechnology | 203 |
DNA in Forensic Science | 295 |
Vaccination and Gene Manipulation | 317 |
Transgenesis | 357 |
Protein Engineering | 379 |
Bioinformatics | 405 |
Immobilization of Biocatalysts | 433 |
Protein Extraction | 461 |
Monoclonal Antibodies | 497 |
Molecular Structural and Chemical Biology | 237 |
Genetically Modified Foods | 261 |
Molecular Diagnosis of Inherited Disease | 279 |
Biosensors | 521 |
| 555 | |
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Common terms and phrases
Acad activity amino acid amplification analysis annealing antibody antigen applications bacteria bacteriophage binding biocatalyst Biol biological biosensor Biotechnol Biotechnology catalytic cDNA cell-lines cells chromatography chromosome cloning vector coding coli containing culture Curr database denatured detected digestion disease DNA fragments DNA polymerase DNA sequence domain electrophoresis epitopes eukaryotic example Figure foreign DNA function fusion gene expression gene library gene probe genetic genome glucose growth heterologous homologous host human hybridization immobilized immune infection insert isolated labelled ligation marker medium membrane method microbial molecular biology molecules mRNA mutation Natl Nucleic Acids nucleotide oligonucleotide organism pathogens PCR products peptide phage phagemid plant plasmid potential primers Proc promoter protease protein purification reaction receptor region replication resistance restriction enzyme sample secondary metabolites selection specific strains strand structure substrate synthesis target techniques tion transcription transfection transgenic vaccine viral virus yeast