Plant Cell Culture

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Garland Science, 2003 - Science - 194 pages
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Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology. This book will be an essential addition to any plant science laboratory's bookshelf.
 

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Contents

Introduction
1
Industrialscale plant cell culture
3
Plant reproductive tissues
9
Tissue culture in agriculturei
19
Biodiversity and conservation of germplasm
27
Applications of plant genetic engineering
39
The basic laboratory layout and equipment
43
Disposing of contaminated waste
53
Genetic transformation of suspension cultured cells
94
Protoplast culture
95
Preparation of protoplasts from suspension cultures
101
Preparation of protoplasts from roots arabidopsis
108
Detecting regeneration of cell wall in isolated
114
Haploid cultures
121
Wheat microspore culture
127
Hairy root cultures plant transformation with
135

Preparation of hormone additions to basal media
60
Monitoring the growth of callus
67
Isolation and culture of the primary seedling root
68
Callus from a dicot root Arabidopsis thaliana
73
Preparation of a transgenic Arabidopsis thaliana callu
79
Uses of cell suspension cultures
87
Embryogenesis from callus in a dicot e g carrot
148
To culture coli in Luria broth LB
162
Industrial uses of plant cell culture
169
Glossary
185
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About the author (2003)

David Evans is the author of the catalogue raisonnA(c) "John Heartfield: AIZ/VI 1930-38" and a Research Fellow in Photography at the Arts Institute, Bournemouth, England. He has published numerous articles in such journals as "Afterimage, Eye, " and "Source.

Anne Kearns trained in psychoanalytic psychotherapy in the USA and in Transactional Analysis and Gestalt Therapy in the UK. She is a former Chair and Teaching and Supervising member of the Gestalt Psychotherapy and Training Institute. In 1999 she founded The Growing Edge, a consortium of psychotherapists and other professionals who are interested in the development of the profession of psychotherapy through post-qualification training and consultation. Anne has an MSc in Integrative Psychotherapy and was Course Director of the training in Integrative Group Therapy at the Metanoia Institute from 1994 96 and a Primary Tutor on the Integrative Psychotherapy training from 1994 1999.

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