Plant Cell CulturePlant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology. This book will be an essential addition to any plant science laboratory's bookshelf. |
Contents
Introduction | 1 |
Industrialscale plant cell culture | 3 |
Plant reproductive tissues | 9 |
Tissue culture in agriculture | 19 |
Biodiversity and conservation of germplasm | 27 |
Tissue culture in genetic | 33 |
Introduction | 39 |
Contamination | 43 |
Protoplast culture | 95 |
Preparation of protoplasts from suspension cultures | 101 |
Preparation of protoplasts from roots arabidopsis | 108 |
Detecting regeneration of cell wall in isolated | 114 |
Haploid cultures | 121 |
Wheat microspore culture | 127 |
Isolation and culture of the primary seedling root | 133 |
9 | 136 |
Disposing of contaminated waste | 53 |
Preparation of hormone additions to basal media | 60 |
Monitoring the growth of callus | 67 |
Callus from a dicot root Arabidopsis thaliana | 73 |
Preparation of a transgenic Arabidopsis thaliana callu | 79 |
Uses of cell suspension cultures | 87 |
Genetic transformation of suspension cultured cells | 94 |
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Common terms and phrases
250-ml conical flask acid agar agarose Agrobacterium antibiotic Arabidopsis aseptic autoclave auxin bacterial basal Bench centrifuge bottles callus cell and tissue cell culture cell division cell suspension cultures cell wall Chapter chromosome coconut commercial compounds concentration containing crops culture medium cytokinin dicots embryogenesis embryogenic embryos enzymes Equipment Materials explants filter fluorescent forceps fusion gene genetic genome grown growth h dark hairy roots haploid hormones incubator induced initiation isolated laboratory laminar flow cabinet liquid maize Materials and reagents meristem methods micropropagation microscope microspores organs pellet Petri dishes pipette plant breeding plant cell plant cell culture plant culture plant material plant tissue culture plantlets plates procedures production propagation proteins protoplasts reagents Protocol Resuspend Scalpel seedlings seeds selection shaking shoots solution somaclonal variation somatic embryogenesis Somatic hybridization species subcultured sucrose techniques tips transfer transformation transgenic plants tubes tumefaciens viable vitamins vitro
References to this book
Cell and Tissue Reaction Engineering Regine Eibl,Dieter Eibl,Ralf Pörtner,Gerardo Catapano,Peter Czermak Limited preview - 2008 |