Plant Tissue Culture: Practices and New Experimental Protocols

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I. K. International Pvt Ltd, 2007 - Botany, Experimental - 316 pages
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Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.
 

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Contents

Historical Developments in Plant Tissue and Cell Culture
1
Laboratory Design and Development
9
Instrumentation
19
Plant Tissue Culture Media
29
Sterilization Techniques
55
Safety Rules and Regulations 65
64
Micropropagation
79
Micropropagation by Proliferation of Axillary Buds
87
Protoplast Isolation and Culture
161
Protoplast Fusion and Cybridization 173
172
In Vitro Germplasm Conservation
183
In Vitro Production of Plant Secondary Metabolites
191
Acclimatization of Micropropagated Plantlets
197
In Vitro and Ex Vitro Rooting of Microshoots
203
Somaclonal and Gametoclonal Variations 213
212
Problems in Plant Tissue Culture
229

Shoot Tips of Gerbera
98
Plant Regeneration from Callus Cultures
109
Plant Regeneration by Somatic Embryogenesis 123
122
Meristem Culture
131
Embryo Culture 149
148
Endosperm Culture
153
Commercial Micropropagation
245
Microscopic and Photographic Techniques
261
Histological Techniques
271
Glossary
275
Appendix
283
Copyright

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