Practical Microbiology

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S. Chand Publishing, 2002 - Science - 397 pages
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FOR LABORATORY STUDENTS OF ALL INDIAN UNIVERSITIES
 

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Practical microbiology written by Professor Dr. R. C. Dubey and Dr. D. K. Maheshwari is a good book for the students of microbiology and biotechnology specially in the field microbial culture.

Contents

Microbiology Laboratory Basic Rules and Requirements 120
9
Methods for Study of Microorganisms 2147
21
Sampling Observation and Staining of Microorganisms 4877
48
Cultivation and Isolation of Microorganisms 78109
78
Method for getting pure culture of photosynthetic bacteria Experiment 6 Isolation and identification of algae from soil
81
Isolation of cyanobacteria from soilwater from a paddy field
82
Isolation of phylloplane microflora
83
Isolation of seed mycoflora
85
Determination of thermal death time TDT of an organism
166
Effect of pH on bacterial growth Experiment 8 Effect of osmotic pressure on bacterial growth
168
Germicidal effect of UV light on bacterial growth UV survival curve Experiment 10 Effect of heavy metals on oligodynamic action of bacteria
169
Effect of different chemicals on growth of bacteria Experiment 12 Filter paper disc method for evaluation of antiseptics
172
Evaluation of alcohol as skin detergent
173
Test for antimicrobial property KirbyBauer method by disc method Experiment 15 Determination of minimum inhibitory concentration MIC of an a...
175
Determination of oxygen requirements of the given bacteria Experiment 18 Measurement of fungal growth by colony diameter method
178
Measurement of fungal growth by biomass mycelial dry weight method
179

Isolation of protozoa from soil Experiment 11 Isolation of yeast from sugarystarchy materials
87
Slime mold culture through moist chamber method
88
Isolation of aquatic fungi by baiting technique Experiment 14 Slide culture technique for studying morphology of fungi
89
Isolation of VAM spores from soil
91
Isolation and enumeration of fungi from soil
93
Isolation and enumeration of bacteria from soil
94
Isolation of actinomycetes from soil
95
Isolation and enumeration of rhizosphere microorganisms
96
Isolation of rhizoplane microorganisms
97
S ratio and assessment of rhizosphere effect Experiment 22 Isolation of Rhizobium from soilroot nodules
98
Isolation of Azotobacter from garden soil
99
Isolation of Azospirillum from soilroots
100
Isolation of fluorescent pseudomonads from potato rhizosphere
101
Identification of mushrooms by spore print method
103
Special Features of Selected Microorganisms 110141
110
Microbial Interactions 142159
142
Demonstration of bacterial antagonism
143
Identification of symbiotic bacteroids of Rhizobium
144
Determination of nitrogen fixing ability of rhizobia in terms of nitrogenase ac tivity
145
Explanta uptake of hydrogenase assay in Rhizobium leguminosarum
146
Determination of leghaemoglobin content from the healthy root nodules of a leguminous plant
147
Measurement of allantoin and allantoic acid in root nodules
148
Nodulation by Rhizobium i e plant infection test and determination of effi ciency of nodulation
149
Microscopic observations of root colonisation by VAM fungi Experiment 11 Histochemical localization of chemicals in endomycorrhizal symbiosis
151
Histochemical localization of lipids in endomycorrhizal roots
152
Histochemical localization of succinate dehydrogenase Experiment 14 Localisation of enzyme alkaline phosphatase and acid thosphatase
153
Histochemical localization of peroxidase Experiment 16 Demonstration of cyanobacterial symbiosis in Azolla
154
Demonstration of lichen symbiosis microscopic observation
156
Microbial Growth 160183
160
Determination of growth curve of bacteria E coli and Pseudomonas Experiment 2 Bacterial population count by turbidimetry determination method ...
164
Determination of thermal death point TDP of an organism
165
Characterisation of Microbial Cultures 184205
184
Sugar mannitol fermentation by bacteria
185
IMVIC test of enteric bacteria
186
Indole production test
189
Oxidase activity of a given bacterial sample Experiment 6 Demonstration of carbohydrate metabolism i e oxidation and fermentation of glucose by H...
191
Determination of nitrate reduction by bacteria
193
Demonstration of starch hydrolysis by a given bacterial culture Experiment 9 Demonstration of protein gelatin hydrolysis
195
Fat hydrolysis lipase activity by a bacterial culture
196
Phenylalanine deaminase production Experiment 12 Demonstration of lysine decarboxylase activity of bacteria
197
Degradation of sulphur containing amino acids e g cysteine and methionine for H₂S production
198
Demonstration of urease production urea hydrolysis Experiment 15 Fermentative production of amylase by Bacillus species
200
Fermentative production of amylase by Aspergillus niger
201
Measurement of enzyme activity of alphaamylase Experiment 18 Effect of substrate concentration on enzyme activity
202
Effect of temperature on enzyme activity
204
Soil Microbiology 206227
206
Quantitative measurement of phosphate solubilisation in culture medium
207
Production of ammonia from organic compounds i e ammonification
208
Bioconversion of ammonia into nitrate i e nitrification Experiment 5 Determination of nitrate production in nitrite broth soil cultures Experiment 5
209
Soil bacteria for denitrification
210
Isolation of an antibiotic producer from soil
211
Determination of antimicrobial spectrum of isolate
212
Isolation and detection of fusaric acid from Fusarium oxysporum Experiment 10 Detection of siderophore produced by a given microorganism Expe...
215
Measurement of microbial activity in soil by soil respiration method
216
Estimation of microbial biomass carbon by chloroformfumigation incubation method CFIM
218
Demonstration of indole acetic acid IAA production by soil fungi I Extraction of IAA from culture filtrate
220
Quantitative measurement of IAA produced by soil fungi in vitro
222
Isolation of fungal pathogens from soildiseased plants
223
Isolation of Fusarium sp from soil II Isolation of Macrophomina phaseolina from soil
224
Isolation of Phytophthora sp from soil
225
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