Proteomics: From Protein Sequence to Function

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Garland Science, 2001 - Medical - 313 pages
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Following the announcement of the draft sequence of the human genome and the completion of many others, attention is now increasingly turning to the analysis of the proteins encoded by genomes - proteomics. This is a rapidly emerging research area and this book reviews recent developments in proteomics and compares them with current genomics technologies. The book is written by experts, from academia and industry, who review their area in a concise yet easy to understand style, providing a comprehensive review volume that integrates genomics with proteomics. This is the first book to address the subject at an undergraduate level.
 

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Contents

Methods for measurement of gene mRNA expression
23
Twodimensional polyacrylamide gel eletrophoresis for proteome analysis
43
Detecting proteins in polyacrylamide gels and on electroblot membranes
65
Eickhoff Holger MaxPlanckInstitute of Molecular Genetics Ihnestrasse
73
Mass spectrometrybased methods for protein identification
87
Evers Stefan F HoffmannLa Roche Ltd PRPID Building 6916 Basel
99
Image analysis of twodimensional gels
131
the impact of stable isotope
151
Conclusions
188
The automation concept
194
Robotics
200
Novel approaches to protein expression analysis
207
Application of proteome analysis to drug development and toxicology
225
Phage antibodies as tools for proteomics
237
Glycobiology and proteomics
257
Proteomics in an academic environment
277

Wilkins Marc Proteome Systems Ltd Locked Bag 2073 North Ryde Sydney
156
technical and informatic solutions for high
171
Informatic solutions for proteomics
181
Proteomics as tool for plant genetics and breeding
289
Index
311
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Page 309 - Klose, J. (1975) Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals.

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