Principles of Gene Manipulation: An Introduction to Genetic Engineering

Acad acid agarose gel ampicillin annealed B-galactosidase B-globin bacterial bacteriophage base pairs CaMV cDNA chromosome cleavage cleaved cloned DNA cloned gene cloning vehicles cohesive ends colicin colonies constructed containing cosmid covalently deletion derived detected digestion DNA fragments DNA ligase DNA molecules DNA polymerase DNA sequences duplex DNA Eco RI Escherichia coli eukaryotic exogenous DNA experiments foreign DNA gel electrophoresis gene manipulation genetic genome Hind HindIII host cell hybrid infected inserted isolated ligated linear lysogen Mdal method Molec molecular mouse mRNA mutants natn nick nopaline nucleotide octopine oligo packaging phage phenotype plant plaques plasmid DNA polypeptides Proc produced promoter protein pSom PstI recombinant DNA recombinant plasmid replication resistance restriction and modification restriction endonuclease S1 nuclease selection single-stranded somatostatin strain strand subtilis SV40 DNA synthesis T4 DNA ligase terminal transcription transferase transformation translation tumour vector DNA viral virus vitro yeast