Nature, Volume 429 |
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Page 87
Moreover , COP1 serves as an E3 ubiquitin ligase for p53 in vitro and in vivo , and inhibits p53 - dependent transcription and apoptosis . Depletion of COP1 by short interfering RNA ( siRNA ) stabilizes p53 and arrests cells in the G1 ...
Moreover , COP1 serves as an E3 ubiquitin ligase for p53 in vitro and in vivo , and inhibits p53 - dependent transcription and apoptosis . Depletion of COP1 by short interfering RNA ( siRNA ) stabilizes p53 and arrests cells in the G1 ...
Page 88
HA - ubiquitin p53 Control COP1 MDM2 Pirh2 WB : COP1 WB : MDM2 ... 0.4 0.2 WB : p53 WB : actin Flag vector I COP1 Flag - COP1 • C C C C Vector + ALLN Flag vector COP1ARING COP1ARING Flag vector COP1 COP1 Flag - COP1ARING p53 COP1ARING P ...
HA - ubiquitin p53 Control COP1 MDM2 Pirh2 WB : COP1 WB : MDM2 ... 0.4 0.2 WB : p53 WB : actin Flag vector I COP1 Flag - COP1 • C C C C Vector + ALLN Flag vector COP1ARING COP1ARING Flag vector COP1 COP1 Flag - COP1ARING p53 COP1ARING P ...
Page 89
which was abrogated when the RING - finger domain on COP1 was deleted ( Fig . 2a ) . Furthermore , COP1 was able to decrease the steady - state levels of endogenous p53 in U2 - OS cells ( Fig . 2b ) . To gain further insight into the ...
which was abrogated when the RING - finger domain on COP1 was deleted ( Fig . 2a ) . Furthermore , COP1 was able to decrease the steady - state levels of endogenous p53 in U2 - OS cells ( Fig . 2b ) . To gain further insight into the ...
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actin activity addition analysis animal applications areas associated authors B-cells biology candidates cells Centre climate close complex containing COP1 core corresponding culture Department detection determine disease drug effects et al example experience expression factor field Figure function funding further gene genetic genome growth human important increase indicate individual Institute interactions interests International involved laboratory leading levels limited materials measured mechanism Methods molecular Nature observed obtained organizer performed plants pollen population position potential predicted present production protein publishing record region regulation response role says Science scientific scientists selection sequence shown signal single species structure successful suggest Supplementary surface temperature transcription University western blot