Nature, Volume 429 |
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Page 273
Cell culture and transfection Cell culture and transfections were performed as described25 . Proportions were scaled up linearly for 150 - mm dishes . For downstream northern , immunoblot or nuclear run - on analysis , cells were ...
Cell culture and transfection Cell culture and transfections were performed as described25 . Proportions were scaled up linearly for 150 - mm dishes . For downstream northern , immunoblot or nuclear run - on analysis , cells were ...
Page 302
Reporter assays - Reporter assays were performed essentially as described previously1 . The reporter construct pGL3 - E - cadh promoter ( 2.5 pg ) , which corresponds to the - 178 to +92 human E - cadherin promoter fragment ...
Reporter assays - Reporter assays were performed essentially as described previously1 . The reporter construct pGL3 - E - cadh promoter ( 2.5 pg ) , which corresponds to the - 178 to +92 human E - cadherin promoter fragment ...
Page 313
N17 were Immunofluorescence microscopy and biochemical assays Immunofluorescence microscopy , immunoprecipitation and immunoblotting were performed as described 12.28 . Cells were lysed on ice in a buffer containing 1 % Triton X - 100 ...
N17 were Immunofluorescence microscopy and biochemical assays Immunofluorescence microscopy , immunoprecipitation and immunoblotting were performed as described 12.28 . Cells were lysed on ice in a buffer containing 1 % Triton X - 100 ...
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Bibliographic information
| Title | Nature, Volume 429 |
| Editor | Sir Norman Lockyer |
| Publisher | Macmillan Journals Limited, 2004 |
| Original from | University of Illinois at Urbana-Champaign |
| Digitized | 20 Dec 2019 |
| Export Citation | BiBTeX EndNote RefMan |