Techniques in Aquatic Toxicology, Volume 2, Volume 2Gary K. Ostrander Following up on his popular Techniques in Aquatic Toxicology with a second volume, now nine years later, Dr. Ostrander has once again called on the top aquatic toxicologists from across the world to present 39 chapters of unique collection and testing procedures. Updating five techniques from the first volume, the authors have gone on to add over two dozen new techniques. Every chapter covers a specific procedure that can easily be reproduced by any competent technician with basic knowledge. Each of the chapter authors provides and interprets typical and anomalous results, false positives, and artifacts. Data is provided either from recently published experiments or from work being published for the first time. |
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acid activity amphipod analysis analyte animals approximately aquatic assay assessment atrazine behavior bioassay bioavailability biodegradation biomarker bivalves buffer calibration cell cellular Chem chemical chinook salmon compounds concentrations contaminants copper coral culture detection determined dichloromethane dilution dissolved DMBA effects effluent eggs embryos endpoints Environ environmental estrogenic ethanol exposed exposure extraction FETAX Figure filter fish flask flow cytometry fluorescence freshwater growth HPLC/PDA ICP-MS incubation juvenile laboratory larvae levels lipid lysosomal marine measured medaka mesh metal method mg/l mummichog mutation NIST organisms PAHs parameters pesticide pipette plate pollutants procedure protein protocol rainbow trout reagents reference toxicant replicates reproductive response rinsed salmon samples seawater sediment species spiggin SPME staining standard steroid stock solution studies sulfate Table tank technique temperature tissue toxicity toxicity tests Toxicol toxicology treatment tube values vials vitellogenin volume week µg/l
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Page 15 - Bradford. MM A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254. 1976.