High-Performance Thin-Layer Chromatography for the Analysis of Medicinal PlantsEike Reich, Anne Schibli High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants presents the theoretical and technical information needed to perform reliable and reproducible high-performance thin-layer chromatography (HPTLC) to establish the identity, purity, quality, and stability of raw materials, extracts, and finished botanical products. The text provides a complete overview of the technique and common applications of HPTLC in herbal analysis. It will help the analyst answer questions such as: |
Contents
1 | |
6 | |
11 | |
19 | |
22 | |
Special Aspects of TLC | 33 |
The Stationary Phase | 42 |
The Mobile Phase | 48 |
Typical Applications in Herbal Analysis | 129 |
HPTLC Analysis of Raw Material | 132 |
HPTLC Analysis of Herbal Drug Preparations and Products | 160 |
Method Development | 175 |
Method Development in Practice | 179 |
Validation | 193 |
Validation of Qualitative HPTLC Methods | 206 |
Validation of Quantitative HTPLC Methods | 210 |
The Chromatographic Result in TLC | 55 |
Densitometry | 61 |
Practical Aspects of Modern TLC | 67 |
Sample Preparation | 68 |
Selecting the Stationary Phase | 73 |
Sample Application | 92 |
Chromatogram Development | 97 |
Derivatization | 110 |
Densitometry | 116 |
Reporting and Record Keeping | 121 |
TLC Software | 125 |
Practical Recommendations | 127 |
The Final Word | 214 |
List of Abbreviations | 215 |
Glossary of TLC Terms | 218 |
Definitions | 224 |
Standard Operating Procedure for HPTLC of Herbal Raw Materials and Preparations | 227 |
Resources | 231 |
Common Mobile Phases for the Screening of Unknown Herbal Drugs | 233 |
Most Common Nonspecific Derivatization Reagents for Analysis of Herbal Drugs | 234 |
Worksheet | 238 |
Validation of Method for Identification of Eleuthero by HPTLC Fingerprint | 240 |
Index | 259 |
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High-performance Thin-layer Chromatography for the Analysis of Medicinal Plants Eike Reich,Anne Schibli No preview available - 2007 |
Common terms and phrases
acetic acid adsorbent adsorption adulterants American Herbal Pharmacopoeia Analysis of Medicinal analytical anisaldehyde anisaldehyde reagent application position band bonded phases CAMAG Chapter 3 Practical chemical chloroform chro chromatogram chromatographic result compounds densitometry derivatization detection developing chamber developing distance developing solvent dichloromethane Eleuthero Eleutheroside ethyl acetate evaluation extract Figure fingerprint flavonoids fluorescence formic acid Herbal Analysis herbal drug hexane HPLC HPTLC HPTLC fingerprints HPTLC plates identification interaction layer markers matogram Medicinal Plants Merck methanol method development migration mixture mobile phase Modern TLC molecules multiple development Natural Products reagent parameters peak polar quantitative raw material relative humidity retention RF values root sample application saturation selectivity separation Siberian ginseng silica gel solvent solvent front solvent strength specific spraying stability tests standard stationary phase suitable sulfuric acid sulfuric acid reagent tion TLC plate toluene validation white light zones