High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants

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Eike Reich, Anne Schibli
Thieme, Jan 1, 2011 - Medical - 280 pages
High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants presents the theoretical and technical information needed to perform reliable and reproducible high-performance thin-layer chromatography (HPTLC) to establish the identity, purity, quality, and stability of raw materials, extracts, and finished botanical products. The text provides a complete overview of the technique and common applications of HPTLC in herbal analysis. It will help the analyst answer questions such as:
  • Am I paying for a high-quality material, but getting a cheap adulterant?
  • Is this raw material worth its price?
  • Does this product comply with the claim on its label?
  • Has the composition of this product changed after being on the shelf for more than a year?
  • Practical examples provided by renowned experts help the reader gain a firm understanding of HPTLC methodologies. More than 300 full-color illustrations aid comprehension of complex concepts, and easy-to-reference text boxes provide summaries of key information.This book is essential for analysts, quality assurance professionals, and regulators seeking a comprehensive text on how to use HPTLC to determine whether botanicals comply with current, good manufacturing practices. It will also benefit students in pharmacognosy, phytopharmacy, pharmaceutical biology, and analytical chemistry programs.
     

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    Contents

    Introduction
    1
    What Is Chromatography?
    6
    What Is TLC?
    11
    Modern TLC
    19
    Theoretical Concepts
    22
    Special Aspects of TLC
    33
    The Stationary Phase
    42
    The Mobile Phase
    48
    Typical Applications in Herbal Analysis
    129
    HPTLC Analysis of Raw Material
    132
    HPTLC Analysis of Herbal Drug Preparations and Products
    160
    Method Development
    175
    Method Development in Practice
    179
    Validation
    193
    Validation of Qualitative HPTLC Methods
    206
    Validation of Quantitative HTPLC Methods
    210

    The Chromatographic Result in TLC
    55
    Densitometry
    61
    Practical Aspects of Modern TLC
    67
    Sample Preparation
    68
    Selecting the Stationary Phase
    73
    Sample Application
    92
    Chromatogram Development
    97
    Derivatization
    110
    Densitometry
    116
    Reporting and Record Keeping
    121
    TLC Software
    125
    Practical Recommendations
    127
    The Final Word
    214
    List of Abbreviations
    215
    Glossary of TLC Terms
    218
    Definitions
    224
    Standard Operating Procedure for HPTLC of Herbal Raw Materials and Preparations
    227
    Resources
    231
    Common Mobile Phases for the Screening of Unknown Herbal Drugs
    233
    Most Common Nonspecific Derivatization Reagents for Analysis of Herbal Drugs
    234
    Worksheet
    238
    Validation of Method for Identification of Eleuthero by HPTLC Fingerprint
    240
    Index
    259
    Copyright

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    About the author (2011)

    Head of Laboratory, CAMAG, Muttenz, Switzerland

    Assistant Head of Quality Control, SwissCo Services AG, Sisseln, Switzerland

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