Genetic Manipulation: Techniques and ApplicationsJ. M. Grange, K. R. Fox, N. L. Morgan Genetic manipulation is no longer the province of the specialized researcher. It is finding widespread application in all fields of medicine and biology. Nevertheless, application of these relatively new techniques to new areas of research is often fraught with unexpected problems and difficulties. Based on the Society for Applied Bacteriology's Autumn 1989 Conference, this unique volume covers a wide and very up-to-date range of techniques used in genetic engineering. These include the isolation and analysis of DNA and RNA from cells and tissues, the selection and use of phage and plasmic vectors for cloning DNA, the cloning procedures, the production and screening of genomic libraries, the production and use of DNA probes, the polymerase chain reaction and the synthesis of 'designer' genes. This volume contains many examples of the applications of the above and other techniques for genetic manipulation, to subjects as diverse as plant pathology, forensic science, bacterial taxonomy, cardiac research, diagnostic microbiology, food hygiene and sewage treatment. |
Contents
| 1 | |
Techniques and Applications Restriction Mapping of DNA | 15 |
Techniques and Applications The Construction and Use of Cloning Vectors | 25 |
Techniques and Applications Adaptor Based cDNA Cloning in the Phage Vectors lgt10 and lgt11 | 37 |
Techniques and Applications Plasmid Profiling and DNADNA Hybridization for Distinguishing Between Mesophilic Aeromonas Bacteria | 55 |
Techniques and Applications Preparation and Screening of Bacterial Genomic Libraries | 71 |
Techniques and Applications DNA Probes for Detection and Identification of Bacteria | 97 |
Techniques and Applications Extraction and Purification of Eukaryotic mRNA | 113 |
Techniques and Applications Analysis of Restriction Fragment Length Polymorphisms in the Study of Bacteria | 227 |
Techniques and Applications The Use of DNA Probes to Detect Selected Bacteria After Their Introduction into a Waste Water Treatment Plant | 245 |
Techniques and Applications The Use of Genetic Manipulation to Study Bacterial Pathogens | 253 |
Techniques and Applications RNA Detection Methods in Basic Cardiac Research | 265 |
Techniques and Applications The Polymerase Chain Reaction and its Application to Basic Research in Molecular Biology | 279 |
Techniques and Applications The Effect of Nutrient Limitation on the Stability and Expression of Recombinant Plasmids | 295 |
Techniques and Applications Largescale Production of Proteins from Recombinant DNA in Escherichia coli | 315 |
Techniques and Applications Stability and Copy Number of Yeast Cloning Vectors | 337 |
Techniques and Applications cDNA Cloning | 129 |
Techniques and Applications Primer Extension Sequencing of RNA Viruses | 149 |
Techniques and Applications Forensic Applications of DNA Profiling | 169 |
Techniques and Applications Application of Nucleic Acid Probes to the Identification of Bacterial Enteric Pathogens | 181 |
Techniques and Applications Quantification of Collagen mRNA Levels in Mammalian Cells | 191 |
Techniques and Applications Detection of Foodborne Bacterial Pathogens by a Colorimetric DNA Hybridization Method | 203 |
Techniques and Applications Use of a DNA Probe for a Viroid in Plant Pathology | 217 |
Techniques and Applications Designer Genes Design and Synthesis Illustrated for the Gene Coding for Human Macrophage Colony Stimulating Factor | 351 |
Techniques and Applications Monitoring Safety in Process Biotechnology | 361 |
Techniques and Applications Patenting in Biotechnology | 377 |
| 388 | |
Techniques and Applications THE SOCIETY FOR APPLIED BACTERIOLOGY TECHNICAL SERIES | 400 |
Other editions - View all
Genetic Manipulation: Techniques and Applications J. M. Grange,K. R. Fox,N. L. Morgan No preview available - 2009 |
Genetic Manipulation: Techniques and Applications J. M. Grange,K. R. Fox,N. L. Morgan No preview available - 1991 |
Common terms and phrases
acetate adaptor agarose gel amplification analysis antibody aqueous assay bacterial Biology blot buffer cDNA cells centrifugation chloroform chromosomal cloning coli concentration containing copy number culture defined denatured detection digestion dilution DNA fragments DNA hybridization DNA probes dNTP EDTA efficient ethanol ethanol precipitation ethidium bromide extraction filter final first gene gene library genetic genomic guanidinium thiocyanate homology host hybridization identification incubated insert isolated labelled ligation linkers membrane method modified molecular molecules mRNA NaCl nucleic acid nucleotide oligonucleotide patent pellet phage phenol phosphate plaques plasmid plasmid DNA plate polymerase polymerase chain reaction precipitation primer procedure profiling protein protocols purification reaction reagents recombinant removed restriction enzyme restriction fragment Resuspend RFLPs RNase room temperature sample sampler screening sodium solution Southern blot species specific strains synthesis technique transposon Tris-HCl tube vector virus volume wash yeast