Microwave Techniques and ProtocolsRichard T. Giberson, Richard S. Demaree The contributors to this manual are uniformly from those labora- ries routinely using microwave technology to facilitate their processing methods in the various fields of microscopy. The methods and results these authors describe are the tangible evidence that microwaves can be used routinely as the basis for improved sample processing for micr- copy applications. These applications include complete sample proce- ing protocols for light and electron microscopy, decalcification, and immunocytochemistry. The overall time savings, ease of use, and qu- ity of results serve as justification for using microwaves in the labo- tory. The question as to whether there is a "microwave effect" is alluded to, but not discussed in any great detail. When the term microwave technology is used, it is generic and intended to mean equipment designed for laboratory versus household use. Microwave Techniques and Protocols is designed for anyone with a background and experience in sample processing for immunocytoch- istry, decalcification, light microscopy, or electron microscopy, and clearly demonstrates that microwave technology has a place in today's laboratory. Richard T. Giberson, Richard S. Demaree Jr., REFERENCES Giberson, R. T. Demaree, R. S., and (1995) Microwave fixation: understa- ing the variables to achieve rapid reproducible results. Microsc Res Tech 32:246-254. Giberson, R. T., Demaree, R. S., Jr., and Nordhausen, R. W. (1997) Four-hour processing of clinical/diagnostic specimens for electron microscopy using microwave technique. J Vet Diagn Invest 9:61-67. Jr. VhD Ms vi Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
Contents
VacuumAssisted Microwave Processing | 13 |
VacuumMicrowave Combination for Processing | 25 |
Basic Procedure for Electron Microscopy Processing | 37 |
Specimen Preparation for ThinSection Electron | 49 |
Microwave Processing of Archived Pathology | 67 |
Microwave Fixation of Rat Hippocampal Slices | 75 |
Microwave Processing Techniques for Biological | 89 |
Microwave Processing of Sediment Samples | 123 |
Microwave Polymerization in Thin Layers | 139 |
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Microwave Techniques and Protocols Richard T. Giberson,Richard S. Demaree Jr No preview available - 2014 |
Common terms and phrases
acetone aldehyde aleurone antigenicity approx beaker capsules cell cold spot containing cover slip cytoplasmic decalcification dehydration Demaree RS Jr Diagn Invest Dvorak EDTA electron microscopy embedding embryos ethanol fixation steps fixative formalin Giberson and Demaree Giberson and R. S. Giberson RT groups hemidesmosomal Henson Histochem infiltration Kok and Boon laminin Leong load cooler Login and Dvorak Login GR membrane methods Microwave fixation microwave irradiation Microwave Techniques MW cavity MW oven MW processing MW-assisted formalin fixation MW-assisted processing neon bulb array Nordhausen Nordhausen RW osmium tetroxide OsO4 paraffin Pelco Petri dish placed plant polymerization preparation preservation procedure proteins Protocols Edited protoplasts R. S. Demaree Jr R. T. Giberson rapid rinse routine sample sections sediment slice Spurr's staining Techniques and Protocols Ted Pella Teflon temp probe tissue tissue processing Totowa transmission electron microscopy ultrastructural vac chamber vac-MW vial water load wattage