Genetic Engineering: Principles and Methods, Band 23Jane K. Setlow Springer Science & Business Media, 31.08.2001 - 232 Seiten Over the past decade, our laboratory and others have been concerned with molecular archaeological studies aimed at revealing the origins and evolutionary histories of permeases (1). These studies have revealed that several different families, defined on the basis of sequence similarities, arose independently of each other, at different times in evolutionary history, following different routes. When complete microbial genomes first became available for analysis, we adapted p- existing software and designed new programs that allowed us quickly to identify probable transmembrane proteins, estimate their topologies and determine the likelihood that they function in transport (2). This work allowed us to expand previously-recognized families and to identify dozens of new families. All of this work then led us to attempt to design a rational but comprehensive classification system that would be applicable to the complete complement of transport systems found in all living organisms (3). The classification system that we have devised is based primarily on mode of transport and energy coupling mechanism, secondarily on molecular phylogeny, and lastly on the substrate specificities of the individual permeases (4). |
Inhalt
EVOLUTION OF TRANSPORT PROTEINS | xviii |
MECHANISMS OF APOPTOSIS REPRESSION 1 | xxviii |
CYTOKINE ACTIVATION OF TRANSCRIPTION | lviii |
VECTOR DESIGN AND DEVELOPMENT OF HOST SYSTEM | 11 |
GENETIC AND BIOCHEMICAL STUDIES ON THE ASSEMBLY OF | 24 |
ENZYME AND PATHWAY ENGINEERING FOR SUICIDE GENE | 90 |
THE ROLE | 53 |
EXPRESSION OF FOREIGN GENES IN TIIE YEAST Pichia pastoris | 115 |
Andere Ausgaben - Alle anzeigen
Häufige Begriffe und Wortgruppen
Acad activity addition allow amino acid analysis appears approaches assembly association binding Biochem Biol budding Cell Chem chromatin cleavage Cloning complex conserved containing core cytoplasmic demonstrated dimers domain effect Engineering enzyme eukaryotes example expression extein factor Figure foreign function further gene Genetic genome glycoproteins glycosylation homologues host human identified indicated initiation intein interaction involved kinase ligation MCM proteins mechanism membrane Molecular motifs mutants Nature observed occurs origin particles pastoris pathway peptide phase phosphorylation Plant position precursor present Proc promoter proteases reaction recently region Regulation release replication residues role SAGE Science selection sequence shown signal similar specific splicing strains structure studies substrate suggests synthesis TAGs techniques transcripts transport vectors Virol virus vitro yeast