The station occupies an irregular quadrilateral plot of ground containing about 1,800 sq. m., situated 150 m. from the present water's edge and 5 m. above sea level. Originally located on the strand, it is now widely separated from the sea by intervening railroad yards. The building is a plain but substantial stone structure of two stories facing the west, with its main axis running north and south. It is of rectangular form (13.3 by 21.5 m.) (figs. 41 and 42), with a long ell (5.18 by 25 m.) facing the walled garden and entrance court. The ground floor (fig. 42) contains corridor and eight rooms, namely, the director's office and laboratory, zoological assistants' laboratory, (Pl. XLIV, A), two general zoological laboratories with simple equipment, a physiological laboratory with special apparatus for the study of electrical stimulation, reagent room, and receiving room. The upper floor (fig. 43) contains the library, chemical (with com

FIG. 41. Basement with aquaria, zoological station at Trieste. A1, aquaria of reenforced concrete with 2 glass panes: A2, cement aquaria on window ledges; 43, step aquaria of stone slabs; A4, large aquaria of cement for fish; As, wooden aquarium rack; 46, large wooden tank for fish; C, cement cistern; f, filters. Courtesy of Professor Cori.

plete photographic equipment) laboratory with hood, chemical table and equipment for the simpler lines of work in physiological chemistry, the botanical laboratory with a large collection of algae in fluids, and an herbarium of over 2,000 sheets representing the local and European marine flora named by eminent specialists, the botanical assistant's laboratory, and three smaller laboratories for investigators. The attic (fig. 44) contains the cistern room, tackle room, and quarters for the scientific assistants, while the basement is given over to the aquaria and storerooms. In the wing of the building are located the engine room, machine shop with lathe, drill, and forge, collection room and shipping room, and the laboratory of the Austrian Malaria Commission, which has its office at the station, and the servants' quarters.

ENTRANCE

The laboratories are supplied with fresh and salt water and compressed air and are heated and lighted by gas. Their equipment is of the simplest sort and although generally ample the rooms are not always well adapted to the uses to which they must be put.

The recently installed aquaria and circulating system have been erected with great care and represent a high grade of simplicity and efficiency. The water is drawn through a sea pipe 150 m. in length, terminating at the water's edge at low-tide level, then connected with the pump of the Argo. The pipe is closed except when pumping

FIG. 42.-Ground or first floor, zoological station at Trieste: a, aquarium racks; experimental table with sea water and compressed air. See scale on figs. 43 and 44. Courtesy of Professor Cori.

is in progress. It is 60 mm. in diameter, iron enameled outside and in. The pump is a one-cylinder Weisse and Monsky, bronze lined, with a capacity of 9 cu. m. per hour. It is run by a 4-horsepower Grob gas motor, a 2-horsepower Otto engine having proved too weak to furnish the supply needed. The water is stored in a cement tank (fig. 41, C) (4 by 7.5 by 1.2 m.), containing 30 cu. m. beneath the floor of the basement, and in high-level iron tanks in the attic, coated with pitch, containing 12 cu. m. The iron tanks (fig. 44) have not

proved to be satisfactory. The water is used in a closed circulation system and is filtered through sand filters (fig. 41, f) after passing through the aquaria before pumping to the high-level tanks. The filters contain quartz sand (1-3 mm. grains) 40 cm. deep. A special reservoir of 5 cu. m. capacity beneath the machine room provides water not in circulation for experimental and shipping purposes. Water is pumped from the sea under favorable conditions of tide and weather and must be renewed at a maximum in three months. The circulating system is of soft lead with 60 mm. mains and branches of 30, 20, and 12 mm. All valves and cocks are of hard rubber.

Compressed air is supplied by a Weisse and Monsky compressor of four atmospheres, and is stored in four steel chambers each 80 cm. in diameter and 200 in length and a total capacity of about 4,500 liters. A vacuum apparatus is also attached to the compressor. Before distribution the pressure of the air is reduced to 1.05 atmospheres by passage through a Dräger reducing valve and then passes through a pressure regulator to the distributing system, which is of ordinary gas pipe. The maximum use of air at the station is about 20,000 liters per day. Two hours' use of the compressor suffices to provide this quantity.