Optical Fluorescence Microscopy: From the Spectral to the Nano DimensionAlberto Diaspro In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation. |
Contents
1 | |
The White Confocal Continuous Spectral Tuning in Excitation and Emission | 37 |
SecondThird Harmonic Generation Microscopy | 55 |
Role of Scattering and Nonlinear Effects in the Illumination and the Photobleaching Distribution Profiles | 75 |
New Analytical Tools for Evaluation of Spherical Aberration in Optical Microscopy | 85 |
Improving Image Formation by Pushing the SignaltoNoise Ratio | 101 |
SiteSpecific Labeling of Proteins in Living Cells Using Synthetic Fluorescent Dyes | 111 |
Imaging Molecular Physiology in Cells Using FRETBased Fluorescent Nanosensors | 131 |
Measuring Molecular Dynamics by FRAP FCS and SPT | 153 |
In VitroIn Vivo Fluctuation Spectroscopies | 164 |
Interference Xray Diffraction from Single Muscle Cells Reveals the Molecular Basis of Muscle Braking | 183 |
Low Concentration Protein Detection Using Novel SERS Devices | 190 |
Near Infrared ThreeDimensional Nonlinear Optical Monitoring of Stem Cell Differentiation | 211 |
A Correlative Microscopy A Combination of Light and Electron Microscopy | 231 |
239 | |
Other editions - View all
Optical Fluorescence Microscopy: From the Spectral to the Nano Dimension Alberto Diaspro No preview available - 2011 |
Optical Fluorescence Microscopy: From the Spectral to the Nano Dimension Alberto Diaspro No preview available - 2014 |
Optical Fluorescence Microscopy: From the Spectral to the Nano Dimension Alberto Diaspro No preview available - 2010 |
Common terms and phrases
acceptor amplitude antibodies aperture axial axis band beam beam splitter Biol biological Biophys bleaching Braeckmans Catanzaro cellular Chem cm¯¹ cm¹ coefficient collagen components confocal microscopy conformational changes detection detector device Diaspro differentiation diffraction diffusion donor dyes dynamics effect electron embryoid bodies embryonic stem cells emission energy field filter fluorescence correlation spectroscopy fluorescence lifetime fluorescence microscopy fluorescent protein fluorescently labeled fluorophores focal plane focused FRET efficiency function green fluorescent protein illumination imaging intensity distribution interaction Italy laser lens light living cells lysozyme Magna Graecia measurements method Microsc molecular molecules motors myosin neuronal nonlinear optical objective observed optical microscopy phase photobleaching photon polarization probes quantum dots Raman refractive index resolution sample scanning scattering second harmonic sensor SERS Sheppard CJR shown in Fig signal Singapore spatial frequency spectral spectrum splitter structure substrate techniques temperature tunable two-photon Università Università di Milano wavelength Wouters