Cytochrome P450: Structure, Mechanism, and BiochemistryPaul R. Ortiz de Montellano In the ten years that have elapsed since the first edition of this book went to press, the cytochrome P450 field has completed the transition to a discipline in which structure and mechanism, even regulation and biological function, are dealt with in molecular terms. The twin forces that have propelled this remarkable progress have been the widespread adoption of molecular biological approaches and the successful application of modem structural techniques. Only a few P450 primary sequences were available in 1985, whereas hundreds of P450 sequences are now available. Site-specific mutagenesis was then mostly a proverbial gleam in the eye of the P450 community, but it is now a standard technique in the research repertoire. The first crystal structure of a cytochrome P450 enzyme had just been solved in 1985 and appeared on the cover of the first edition. Today, the high-reso lution crystal structures of four soluble bacterial P450 enzymes are available and the race is on to develop approaches that will permit us to determine the structures of the membrane-bound forms of the enzyme. The past ten years has seen phenomenal progress let us hope that the next ten will prove equally exciting. The book is informally divided into four sections. In order to hold the book close to the advancing front of research, some of the chapter topics from the first edition have been dropped to make room for new or expanded topics. |
Other editions - View all
Cytochrome P450: Structure, Mechanism, and Biochemistry Paul R. Ortiz de Montellano Limited preview - 2013 |
Cytochrome P450: Structure, Mechanism, and Biochemistry Paul R. Ortiz de Montellano No preview available - 2010 |
Common terms and phrases
Acad active site amino acid antibodies arachidonic aromatase aromatic atom Biochem Biochemistry Biol Biophys biosynthesis bond bovine camphor carbon catalytic activity cation cDNA cells characterization Chem cholesterol cleavage cloning complex compounds crystal structure cytochrome P450 reductase debrisoquine domain drug metabolism electron transfer enzyme epoxidation expression fatty acid formation function gene Guengerich heme hemoprotein hepatic human cytochrome human liver hydrogen hydroperoxide hydrophobic hydroxylation inactivation induced inhibition inhibitors interactions intermediate involved iron isotope effects ligand liver liver cytochrome liver microsomes mechanism mechanism-based membrane metabolite microsomal microsomal P450s molecular molecule monooxygenase mutagenesis mutant NADPH Natl olefins Ortiz de Montellano oxidation oxygen P450 enzymes P450-catalyzed pathway peptide peroxidase peroxide Pharmacol porphyrin prosthetic heme protein purified putidaredoxin radical rat liver reaction receptor redox region regulation residues role sequence side chain site-directed Sligar species steroid hydroxylase studies substrate substrate binding synthase testosterone transcription