High-performance Thin-layer Chromatography for the Analysis of Medicinal PlantsWith practical examples of how to establish the identity and quality of botanical products High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants presents the theoretical and technical information needed to perform reliable and reproducible high-performance thin-layer chromatography (HPTLC) to establish the identity, purity, quality, and stability of raw materials, extracts, and finished botanical products. The text provides a complete overview of the technique and common applications of HPTLC in herbal analysis. It will help the analyst answer questions such as:
Practical examples provided by renowned experts help the reader gain a firm understanding of HPTLC methodologies. More than 300 full-color illustrations aid comprehension of complex concepts and easy-to-reference text boxes provide summaries of key information. This book is essential for analysts, quality assurance professionals, and regulators seeking a comprehensive text on how to use HPTLC to determine whether botanicals comply with current, good manufacturing practices. It will also benefit students in pharmacognosy, phytopharmacy, pharmaceutical biology, and analytical chemistry programs. |
Contents
1 | |
6 | |
11 | |
19 | |
22 | |
Special Aspects of TLC | 33 |
The Stationary Phase | 42 |
The Mobile Phase | 48 |
Typical Applications in Herbal Analysis | 129 |
HPTLC Analysis of Raw Material | 132 |
HPTLC Analysis of Herbal Drug Preparations and Products | 160 |
Method Development | 175 |
Method Development in Practice | 179 |
Validation | 193 |
Validation of Qualitative HPTLC Methods | 206 |
Validation of Quantitative HTPLC Methods | 210 |
The Chromatographic Result in TLC | 55 |
Densitometry | 61 |
Practical Aspects of Modern TLC | 67 |
Sample Preparation | 68 |
Selecting the Stationary Phase | 73 |
Sample Application | 92 |
Chromatogram Development | 97 |
Derivatization | 110 |
Densitometry | 116 |
Reporting and Record Keeping | 121 |
TLC Software | 125 |
Practical Recommendations | 127 |
The Final Word | 214 |
List of Abbreviations | 215 |
Glossary of TLC Terms | 218 |
Definitions | 224 |
Standard Operating Procedure for HPTLC of Herbal Raw Materials and Preparations | 227 |
Resources | 231 |
Common Mobile Phases for the Screening of Unknown Herbal Drugs | 233 |
Most Common Nonspecific Derivatization Reagents for Analysis of Herbal Drugs | 234 |
Worksheet | 238 |
Validation of Method for Identification of Eleuthero by HPTLC Fingerprint | 240 |
Index | 259 |
Other editions - View all
High-performance Thin-layer Chromatography for the Analysis of Medicinal Plants Eike Reich,Anne Schibli No preview available - 2007 |
Common terms and phrases
References to this book
Thin Layer Chromatography in Phytochemistry Monika Waksmundzka-Hajnos,Joseph Sherma,Teresa Kowalska Limited preview - 2008 |
Thin Layer Chromatography in Phytochemistry Monika Waksmundzka-Hajnos,Joseph Sherma,Teresa Kowalska Limited preview - 2008 |