Nature, Volume 428Sir Norman Lockyer Macmillan Journals Limited, 2004 - Electronic journals |
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Page 191
... Cdh1 . After transfection with siRNA , prometaphase HeLa cells were collected and replated in fresh medium . Cells transfected with siRNA targeting Cdh1 showed no G1 - specific reduction in Skp2 and Cks1 , a much less pronounced ...
... Cdh1 . After transfection with siRNA , prometaphase HeLa cells were collected and replated in fresh medium . Cells transfected with siRNA targeting Cdh1 showed no G1 - specific reduction in Skp2 and Cks1 , a much less pronounced ...
Page 192
... Cdh1 Cdc20 Skp2 + Cdh1 Skp2 + Cdc20 IB : Skp2 IB : Cdh1 / Cdc20 ( anti - HA ) Skp2 Cdh1 / Cdc20 ( anti - HA ) Lysate IP : anti - HA Skp2 WT Skp2 D - box I mutant Skp2 ( 494 ) Skp2 ( A45 ) 0 12 24 36 0 12 24 36 + Cdh1 Skp2 ( anti - Flag ) ...
... Cdh1 Cdc20 Skp2 + Cdh1 Skp2 + Cdc20 IB : Skp2 IB : Cdh1 / Cdc20 ( anti - HA ) Skp2 Cdh1 / Cdc20 ( anti - HA ) Lysate IP : anti - HA Skp2 WT Skp2 D - box I mutant Skp2 ( 494 ) Skp2 ( A45 ) 0 12 24 36 0 12 24 36 + Cdh1 Skp2 ( anti - Flag ) ...
Page 196
... Cdh1 Mock Control Skp2- Cdk2 Luc Cdh1-199 Cdh1-127 - - --- Cdh1-427 Cdh1-166 Cdh1-463 Tubulin Control siRNA b Time after nocodazole release : Control siRNA 0 2 4 6 9 12 15 18 21 Cdh1 Skp2 p27 Cdc20 Pik Cdh1-199 siRNA Cyclin A 2852 18 15 ...
... Cdh1 Mock Control Skp2- Cdk2 Luc Cdh1-199 Cdh1-127 - - --- Cdh1-427 Cdh1-166 Cdh1-463 Tubulin Control siRNA b Time after nocodazole release : Control siRNA 0 2 4 6 9 12 15 18 21 Cdh1 Skp2 p27 Cdc20 Pik Cdh1-199 siRNA Cyclin A 2852 18 15 ...
Contents
of genetically modified grain | 8 |
Just | 14 |
Medical editors urged to accept ethical code | 17 |
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activity analysis antibody apoptosis applications assays atom Biol biology boson cancer candidates Cdh1 cell biology Centre China Chlb chromosome Cks1 clinical cloned complex culture cyclin cyclin E density disease domain drug e-mail effects experience Ferroplasma follicle function funding gene expression genetic genome GlcN6P hCDC4 Higgs boson human increase indicated infection ING4 Institute interactions kinetochores Laboratory mice microtubules molecular molecules mucus mutations myocardin Nature nature publishing group Naturejobs neurons observed ocean oocytes ovaries phase photon Phys polar population postdoctoral prion probe production protein PSI+ qubit rapamycin receptor recombination region riboswitches ribozyme rld1 RNA interference role scientific scientists sequence signal siRNA Skp2 species stem cells strains structure Supplementary Fig surface target temperature transfected tubulin tumour University wild-type yeast