Nature, Volume 428Sir Norman Lockyer Macmillan Journals Limited, 2004 - Electronic journals |
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Page 191
... Cdh1 expression in T98G cells exiting the cell cycle ( as shown by the accumulation of p27 ; Fig . 2c ) . Silencing of Cdh1 prevented the degradation of Skp2 and Cks1 for up to 36 h after serum removal , when the effect of the siRNA ...
... Cdh1 expression in T98G cells exiting the cell cycle ( as shown by the accumulation of p27 ; Fig . 2c ) . Silencing of Cdh1 prevented the degradation of Skp2 and Cks1 for up to 36 h after serum removal , when the effect of the siRNA ...
Page 192
... Cdh1 Cdc20 + Cks1 Cdh1 + Cks1 Cdc20 f WT Skp2 ( 194 ) Skp2 ( 445 ) p27 Skp1 Skp2 WT -Cdh1 + Cdh1 C WT + S41E + N45R E63Q D - box mutant Cdh1 Cks1 ( anti - Flag ) Cdh1 ( anti - HA ) Skp1 Skp2 WT Skp2 ( 194 ) IB : Skp2 IB : Cdh1 / Cdc20 ...
... Cdh1 Cdc20 + Cks1 Cdh1 + Cks1 Cdc20 f WT Skp2 ( 194 ) Skp2 ( 445 ) p27 Skp1 Skp2 WT -Cdh1 + Cdh1 C WT + S41E + N45R E63Q D - box mutant Cdh1 Cks1 ( anti - Flag ) Cdh1 ( anti - HA ) Skp1 Skp2 WT Skp2 ( 194 ) IB : Skp2 IB : Cdh1 / Cdc20 ...
Page 196
... Cdh1 Skp2 p27 Plk Cdk2 nocodazole release : 0 4 8 12 16 0 4. a RNAi treatment : Cdh1 Skp2 Cdk2 Tubulin Mock Control Luc Cdh1-199 Cdh1-127 Cdh1-166 Cdh1-427 Cdh1-463 C Control siRNA 02 Cdh1-199 siRNA 21 18 15 12 964 Time ( h ) Control siRNA ...
... Cdh1 Skp2 p27 Plk Cdk2 nocodazole release : 0 4 8 12 16 0 4. a RNAi treatment : Cdh1 Skp2 Cdk2 Tubulin Mock Control Luc Cdh1-199 Cdh1-127 Cdh1-166 Cdh1-427 Cdh1-463 C Control siRNA 02 Cdh1-199 siRNA 21 18 15 12 964 Time ( h ) Control siRNA ...
Contents
Ecologists highlight | 19 |
journal editors on the spot | 25 |
Sequencing | 38 |
Copyright | |
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activity analysis antibody apoptosis applications assays atom Biol biology Bmi1 boson cancer Cdh1 cell biology Centre China Chlb chromosome Cks1 clinical cloned complex culture cyclin cyclin E density disease domain drug e-mail effects experience Ferroplasma follicle fracture function funding gene expression genetic genome glms hCDC4 Higgs boson human increase indicated infection ING4 Institute interactions kinetochores Laboratory macrophage mice microtubules molecular mucus mutations myocardin Nature nature publishing group Naturejobs neurons NF-kB observed ocean oocytes ovaries phase photon Phys polar population postdoctoral prion probe production protein PSI+ qubit rapamycin receptor recombination region ribozyme rld1 RNA interference scientific scientists sequence signal siRNA Skp2 species stem cells strains structure Supplementary Fig surface target temperature transfected tubulin tumour University wild-type yeast