Manual of Histologic Staining Methods of the Armed Forces Institute of PathologyPreparation of tissues; Processing of tissue; Preparation of sections; Routine staining procedures; Special techniques; Methods for connective tissue; Methods for connective tissue; Methods for cytoplasmic granules; Methods for hematologic and nuclear elements; Methods for fats and lipids; Methods for carbohydrates and mucoproteins; Methods for pigments and minerals; Methods for nerve cells and fibers; Methods for bacteria, fungi, and inclusion bodies. |
Contents
Preparation of Tissues | 1 |
Processing of Tissue | 12 |
Preparation of Sections | 21 |
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100.0 ml Distilled 15 minutes 24 hours 30 minutes absolute alcohol ACID SOLUTION allow ammonium aqueous Background block blue solution bone buffered neutral formalin carbonate cells clear in xylene color complete container Counterstain crystals Cut paraffin sections Cytoplasm dark decalcifying Dehydrate in 95 demonstration Deparaffinize and hydrate desired Differentiate Dissolve drops eosin excess fibers filter FIXATION fixative FUCHSIN SOLUTION Glacial acetic acid gm Distilled green heat HEMATOXYLIN SOLUTION hydrate to distilled Hydrochloric acid hydroxide iron knife Laboratories light METHOD microns ml Distilled water ml STAINING PROCEDURE Mount with Permount Note Nuclei orange overnight Permount or Histoclad pink Place potassium prepared processing REFERENCE REMARKS remove RESULTS Rinse in distilled running water seconds SILVER NITRATE SOLUTION slide Sodium Sodium thiosulfate SOLUTION See page SOLUTION STOCK specimen STAINING PROCEDURE step sulfate tap water TECHNIQUE three changes tion tissue violet Wash in running Wash in tap yellow