Manual of Histologic Staining Methods of the Armed Forces Institute of PathologyLee G. Luna Preparation of tissues; Processing of tissue; Preparation of sections; Routine staining procedures; Special techniques; Methods for connective tissue; Methods for connective tissue; Methods for cytoplasmic granules; Methods for hematologic and nuclear elements; Methods for fats and lipids; Methods for carbohydrates and mucoproteins; Methods for pigments and minerals; Methods for nerve cells and fibers; Methods for bacteria, fungi, and inclusion bodies. |
Contents
Contents | 1 |
Methods for Fats and Lipids | 140 |
Methods for Carbohydrates and Mucoproteins | 153 |
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100.0 ml Distilled 15 minutes 24 hours 30 minutes absolute alcohol acetic acid solution Alcian blue Aniline aqueous Armed Forces Institute block bone BUFFER SOLUTION buffered neutral formalin celloidin cells clear in xylene Copyright by Williams Counterstain crystals Cut paraffin sections Cytoplasm decalcifying Dehydrate in 95 Deparaffinize and hydrate Differentiate dips Distilled water 100.0 eosin fibers FIXATION fixative frozen sections Glacial acetic acid gm Distilled hydrate to distilled Hydrochloric acid Institute of Pathology knife lithium carbonate METHOD Methyl microns ml Distilled water ml Glacial acetic ml STAINING PROCEDURE Mount with Permount nitrocellulose Nuclei overnight Permount or Histoclad Phloxine Phosphomolybdic acid Phosphotungstic acid picric acid pink Place Potassium ferrocyanide REFERENCE remove RESULTS Rinse in distilled running water Silver nitrate SILVER NITRATE SOLUTION Sodium thiosulfate solution for 30 SOLUTION STOCK specimen sulfate tap water TECHNIQUE three changes tissue Wash in running Wash in tap WEIGERT'S IRON HEMATOXYLIN Williams and Wilkins yellow