NatureSir Norman Lockyer Macmillan Journals Limited, 2007 - Science |
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Page 333
... ubiquitin chains at the catalytic cysteine of Ube2g2 . The growth of Ube2g2 - anchored ubiquitin chains seems to be mediated by an aminolysis - based transfer reaction between two Ube2g2 molecules that each carries a ubiquitin moiety in ...
... ubiquitin chains at the catalytic cysteine of Ube2g2 . The growth of Ube2g2 - anchored ubiquitin chains seems to be mediated by an aminolysis - based transfer reaction between two Ube2g2 molecules that each carries a ubiquitin moiety in ...
Page 334
... ubiquitin chains may be first assembled on Ube2g2 before being transferred to HERPC . Consistent with this idea , fewer Ube2g2 - linked ubiquitin chains were generated when wild - type HERPC was present compared with those formed in the ...
... ubiquitin chains may be first assembled on Ube2g2 before being transferred to HERPC . Consistent with this idea , fewer Ube2g2 - linked ubiquitin chains were generated when wild - type HERPC was present compared with those formed in the ...
Page 336
... Ubiquitin transfer assay . To monitor the transfer of ubiquitin between two Ube2g2s , Ube2g2 ( 1 μM ) was incubated with E1 ( 60 nM ) , Flag - tagged UbK48R or untagged wild - type ubiquitin ( 1 μM ) at 25 ° C in buffers containing ...
... Ubiquitin transfer assay . To monitor the transfer of ubiquitin between two Ube2g2s , Ube2g2 ( 1 μM ) was incubated with E1 ( 60 nM ) , Flag - tagged UbK48R or untagged wild - type ubiquitin ( 1 μM ) at 25 ° C in buffers containing ...
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activity analysis antibodies applications areas assay atoms Biol biology brassinosteroid Ca2+ cancer candidates cell biology Centre channels chemical clinical clone Closing date complex crystal detection disease disorder Drosophila drug electron evolution experience expression field function funding gating pore gene genetic genome global graphene HERPC histone human hybrid hybrid speciation Hydatellaceae imaging indicate Institute interaction interneurons kinase Kuiper belt Laboratory laser lattice Lett Meckel's cartilage Medical Medicine membrane mice microscopy molecular mutations Nature Publishing Group Naturejobs neurons Neuroscience Nymphaeales observed pathway phenotype pheromone photon Phys plants polyhedra polyploid position postdoctoral programme protein pseudovirus quantum rapamycin receptor region role samples says Science scientific scientists sequence signalling silicon species stem cells STIM1 structure Supplementary Fig Supplementary Information surface telomere tion ubiquitin University wild-type Yanoconodon zone