Hairy RootsHairy roots are plant roots that have been genetically transformed and can be cultured on a large scale to replace whole plants for investigating plant secondary metabolism and its genetic manipulation, producing foreign proteins, propagating plants in agriculture, environmental research, and developing new engineering technology for the large-scale production of plant chemicals. The 21 papers cover culture and synthesis, plant propagation, and environmental and bioprocessing aspects. They include detailed instructions for experimental procedures and review recent developments with an emphasis on the cross-disciplinary nature of the research and the direction of future developments. Annotation copyrighted by Book News, Inc., Portland, OR |
Contents
Studies of Secondary Metabolic Pathways in Transformed Roots | 2 |
Accumulation of Cadmium Ions by Hairy Root Cultures | 12 |
Metabolic Engineering of Terpenoid Indole and Quinoline Alkaloid Biosynthesis | 32 |
Characteristics of Selected Hairy Root Lines of Catharanthus roseus | 51 |
Alteration of Metabolite Formation and Morphological Properties of Hairy Roots | 65 |
Production of Ginsenosides in Ginseng Hairy Root Cultures | 73 |
CoCulture of Hairy Roots and Shooty Teratomas | 85 |
Transgenic Crop Plants Using Agrobacterium rhizogenesMediated Transformation | 99 |
Artificial Seed Production through Hairy Root Regeneration | 111 |
BIOPROCESSING ASPECTS OF HAIRY ROOTS | 139 |
Mathematical Modelling of Hairy Root Growth | 169 |
LaboratoryScale Studies of Nutrient Mist Reactors for Culturing Hairy Roots | 191 |
Application of Fungal Elicitation for Enhancing Production of Secondary Metabolites | 209 |
Hairy Roots of Tagetes in TwoPhase Systems | 227 |
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Common terms and phrases
accumulation acid Agrobacterium rhizogenes ajmalicine alkaloid production anabasine auxin belladonna binary vector Bioeng biomass bioreactor biosynthesis Biotechnol buffer cadaverine callus Catharanthus roseus Centrifuge Christey concentration containing cultures of Panax Datura dry weight Duboisia effect elicitor enzymes extraction Figure foreign genes formation fresh weight Furuya genetic ginseng hairy root growth rate hairy root clones hairy root cultures Hamill heterotrophic hybridisation hyoscyamine increased indole alkaloid infection inoculation liquid membrane metabolism mg L-¹ mist MJC Rhodes mL-¹ Nicotiana nicotine NPTII nutrient oxygen Panax ginseng pathways phenotype photoheterotrophy Phytochemistry Plant Cell Plant Cell Rep plantlets plasmid putrescine reactor regenerated Ri plasmid RJ Robins root lines saponin saponin content saponin production scopolamine secondary metabolites shake flasks shikonin shoots shooty teratomas studies sucrose sucrose levels T-DNA tabacum tabersonine Tepfer teratomas tion tips tobacco total antibody transformed root cultures transgenic plants tropane tropane alkaloids tube tures vitro Yoshikawa