Veterinary Microbiology and Microbial Disease

Print version:

Cover; Title page; Copyright page; Dedication page; Preface; Acknowledgements; Author biographies; Section I Introduction to Microbiology Infection Immunity and Molecular Diagnostic Methods; 1 Microbiology microbial pathogens and infectious disease; Further reading; 2 Subdivisions classification and morphological characterization of infectious agents; Microscopical techniques; Pathogenic microorganisms; Biological classification and nomenclature; References; Further reading; 3 Infection and immunity; Normal flora; Comparative aspects of innate and adaptive immunity; Recognition of pathogens. Cells involved in innate immune responsesComplement; Adaptive immunity; Immunity to bacteria; Immunity to fungi; Immunity to viruses; Concluding comments; References; Further reading; 4 Immunodeficiency diseases; Severe combined immunodeficiency diseases; Thymic aplasia or hypoplasia; Wiskott-Aldrich syndrome; Primary immunodeficiency diseases involving B lymphocytes; Secondary immunodeficiency; Further reading; 5 Vaccines and vaccination; Vaccination; Inactivated vaccines; Live attenuated vaccines; Vaccines produced by recombinant nucleic acid technology; Synthetic peptide vaccines. DNA vaccinesReverse vaccinology; Adjuvants; Administration of vaccines; Adverse reactions following vaccination; Vaccination failure; References; Further reading; 6 Molecular diagnostic methods; Analytical properties of nucleic acid; Molecular hybridization; DNA sequencing; Polymerase chain reaction (PCR); Real-time PCR; Diagnostic approaches to surveillance and subtyping of bacteria a comparison of phenotyping and genotyping methods; Molecular subtyping for definitive identification of bacteria; Plasmid profiling; Restriction endonuclease analysis (REA); Ribotyping. Pulsed-field gel electrophoresis (PFGE)PCR-based subtyping methods; Multilocus sequence typing (MLST); DNA microarray technology; Whole bacterial genome sequencing; References; Further reading; Section II Introductory Bacteriology; 7 The structure of bacterial cells; Capsule; Cell wall; Cytoplasmic membrane; Cytoplasm; Ribosomes; Nuclear material; Flagella; Pili; Biofilms; Protein secretion systems; Endospores; Reference; Further reading; 8 Cultivation preservation and inactivation of bacteria; Bacterial growth; Bacterial nutrition; Physical and chemical factors which influence growth. Preservation of microorganismsPhysical methods for inactivating microorganisms; Biosafety cabinets; Reference; Further reading; 9 Bacterial genetics mechanisms of genetic variation and gene databases; Replication of bacterial DNA; Transcription and translation the expression of genetic information; Mechanisms contributing to genetic variation; Examples of mobile genetic elements; Genetic engineering of bacteria in the laboratory; Genetic databases and bioinformatics; Further reading; 10 Laboratory diagnosis of bacterial disease; Selection collection and transportation of specimens